State Key Laboratory of Developmental Biology of Freshwater Fish, Hunan Normal University, Changsha, 410081, Hunan, People's Republic of China.
School of Life Sciences, Hunan Normal University, Changsha, 410081, Hunan, People's Republic of China.
Mar Biotechnol (NY). 2020 Jun;22(3):443-455. doi: 10.1007/s10126-020-09964-5. Epub 2020 Apr 20.
Previous studies have suggested that artificially induced triploid zebrafish are exclusively male-biased. Owing to greatly inhibited gonadal development for the artificially induced triploid fish, they are regarded to be sterile in general. In this article, partially fertile bisexual triploid zebrafish are produced by suppressing extrusion of the second polar body by heat shock. Histological observation confirms that the early gonadal development of these triploid zebrafish is normal. Backcrossing and self-crossing are used to demonstrate that both the female and male triploid zebrafish have partial reproductive ability. Their dynamic of chromosomes during meiosis is revealed from the chromosome preparations of gonads. Examination of the expressed gonadal development-related genes shows some molecular evidence of the normal gonadal development in the triploid zebrafish. Clearly, these fertile bisexual triploid zebrafish can provide a unique system to study sex determination, as well as aneuploidy associated human diseases such as infertility and pregnancy loss.
先前的研究表明，人工诱导的三倍体斑马鱼雄性偏多。由于人工诱导的三倍体鱼类的性腺发育受到极大抑制，通常被认为是不育的。在本文中，通过热休克抑制第二极体的排出，产生了部分可育的两性三倍体斑马鱼。组织学观察证实，这些三倍体斑马鱼的早期性腺发育正常。通过回交和自交证明，雌性和雄性三倍体斑马鱼都具有部分生殖能力。通过对性腺染色体的制备揭示了它们在减数分裂过程中的染色体动态。对性腺发育相关基因的表达检测表明，三倍体斑马鱼的性腺发育存在一些分子证据。显然，这些可育的两性三倍体斑马鱼可以提供一个独特的系统来研究性别决定，以及与不育和妊娠丢失等人类疾病相关的非整倍体。
