Lu Yongqu, Liu Zhenzhen, Wang Wendong, Chen Xin, Zhou Xin, Fu Wei
Department of General Surgery, Peking University Third Hospital, Beijing, China.
Front Med (Lausanne). 2022 Jan 20;8:775357. doi: 10.3389/fmed.2021.775357. eCollection 2021.
The AT-rich interactive domain (ARID) gene family of 15 proteins has an important role in development and proliferation. Gene expression alterations of the ARID family are correlated with the pathogenesis of digestive cancer, but systematic research has not been conducted.
We obtained transcriptome sequencing data, clinical characteristics and stemness indices of the seven main types of digestive cancer (cholangiocarcinoma, colon adenocarcinoma, oesophageal carcinoma, liver hepatocellular carcinoma, pancreatic adenocarcinoma, rectum adenocarcinoma and stomach adenocarcinoma) from public pan-cancer data to combine the analysis of the expression and prognostic signature of the ARID gene family. The stromal and immune scores for each sample were calculated to explore the correlations between the ARID gene family members and the tumour microenvironment.
After screening, 1,920 digestive cancer samples were included in our study. was expressed at low levels throughout the digestive cancer samples. The expression levels of and were relatively high, but there was striking heterogeneity across the different cancer types for specific family members. The survival analysis indicated that many genes were significantly related to the prognosis of patients with liver hepatocellular carcinoma. The stemness indices, stromal score, and immune score analysis showed that the expression of a single ARID gene had characteristic consistency in each tumour, but the levels among the different genes still varied.
Our systematic study of the ARID gene family and its association with the immune infiltrate, tumour microenvironment and outcomes of digestive cancer patients focus on the complex relations and indicate the need to study each ARID member as an individual in a specific cancer type.
由15种蛋白质组成的富含AT的相互作用结构域（ARID）基因家族在发育和增殖中起重要作用。ARID家族的基因表达改变与消化系统癌症的发病机制相关，但尚未进行系统研究。
我们从公共泛癌数据中获取了七种主要消化系统癌症（胆管癌、结肠腺癌、食管癌、肝细胞肝癌、胰腺腺癌、直肠腺癌和胃腺癌）的转录组测序数据、临床特征和干性指数，以联合分析ARID基因家族的表达和预后特征。计算每个样本的基质和免疫评分，以探索ARID基因家族成员与肿瘤微环境之间的相关性。
筛选后，1920例消化系统癌症样本纳入我们的研究。 在整个消化系统癌症样本中表达水平较低。 和 的表达水平相对较高，但特定家族成员在不同癌症类型之间存在显著异质性。生存分析表明，许多基因与肝细胞肝癌患者的预后显著相关。干性指数、基质评分和免疫评分分析表明，单个ARID基因的表达在每种肿瘤中具有特征一致性，但不同基因之间的水平仍有所不同。
我们对ARID基因家族及其与免疫浸润、肿瘤微环境和消化系统癌症患者预后的关联进行的系统研究聚焦于复杂关系，并表明需要在特定癌症类型中将每个ARID成员作为个体进行研究。
