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通过高通量测序检测养殖环境中细菌群落的季节性演替。

Seasonal succession of bacterial communities in cultured detected by high-throughput sequencing.

作者信息

Pang Meixia, Huang Zhili, Lv Le, Li Xiaodong, Jin Gang

机构信息

Postdoctoral Innovation Practice Base, Shenzhen Polytechnic, Shenzhen 518055, China.

School of Applied Chemistry and Biological Technology, Shenzhen Polytechnic, Shenzhen 518055, China.

出版信息

Open Life Sci. 2022 Jan 28;17(1):10-21. doi: 10.1515/biol-2022-0001. eCollection 2022.

Abstract

An increasing number of microorganisms are being identified as pathogens for diseases in macroalgae, but the species composition of bacteria related to , fresh edible green macroalgae worldwide, remains largely unclear. The bacterial communities associated with were investigated by high-throughput 16S rDNA sequencing, and the bacterial diversities in washed and control groups were compared in this study. A total of 4,388 operational taxonomic units were obtained from all the samples, and the predominant prokaryotic phyla were , , , , , , , and in . The bacterial diversity changed with seasons and showed an increasing trend of diversity with the rising temperature in . There were slight reductions in the abundance and diversity of bacteria after washing with tap water for 2 h, indicating that only parts of the bacterial groups could be washed out, and hidden dangers in still exist. Although the reduction in the abundance of some bacteria revealed a positive significance of washing with tap water on food safety, more effective cleaning methods still need to be explored.

摘要

越来越多的微生物被鉴定为大型藻类疾病的病原体,但与全球新鲜可食用绿色大型藻类相关的细菌物种组成仍 largely不清楚。本研究通过高通量16S rDNA测序调查了与 相关的细菌群落,并比较了洗涤组和对照组中的细菌多样性。所有样本共获得4388个可操作分类单元, 中主要的原核生物门为 、 、 、 、 、 、 和 。细菌多样性随季节变化,在 中随温度升高呈现多样性增加趋势。用自来水冲洗2小时后,细菌的丰度和多样性略有降低,表明只有部分细菌群可以被冲洗掉, 中仍存在隐患。虽然一些细菌丰度的降低表明用自来水冲洗 对食品安全具有积极意义,但仍需要探索更有效的清洁方法。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/0ab2/8800382/86009c478f30/j_biol-2022-0001-fig001.jpg

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