Arenajo Althea R, Ybañez Adrian P, Ababan Maria Maichol P, Villajuan Charlotte E, Lasam May Rose M, Young Chiqui P, Reyes Julie Lynn A
College of Pharmacy, University of Southern Philippines-Foundation, Salinas Drive, Lahug, Cebu City 6000, Philippines.
Department of Biology and Environmental Science, College of Science, University of the Philippines Cebu, Lahug, Cebu City 6000, Philippines.
Int J Health Sci (Qassim). 2017 Jul-Sep;11(3):29-32.
Anticoagulants have been used in the treatment of several circulatory diseases and thrombotic disorders, and in the blood sampling for hematologic analysis. Sulfated polysaccharides (SP), which have anticoagulant properties, are found in most seaweeds, including spp.
The study generally aimed to evaluate the potential anticoagulant property of .
The whole plant of fresh was washed thoroughly with distilled water and manually expressed to obtain the extract. extract was tested in two phases. Phase one utilized nine male albino rabbits, which were randomly and equally allocated into three groups: (1) negative control (oral distilled water and subsequent mixing of extracted blood with normal saline solution), (2) positive control (oral aspirin and subsequent mixing of extracted blood with normal saline solution), and (3) experimental group (oral distilled water and mixing of extracted blood with extract). Blood coagulation was evaluated by measuring the clotting time using the slide and tube methods. In phase two, peripheral blood from three apparently healthy adult dogs were used. Blood collection was performed thrice. In each collection, the sample was divided into five aliquots: (1) negative control (normal saline solution), (2) positive control (ethylenediaminetetraacetic acid [EDTA]), and (3-5) experimental treatments at 0.1, 0.15, and 0.2 ml of extract. Coagulation was evaluated by measuring the clotting time using the tube method.
Phase one results revealed significant differences on the clotting time between the negative and the positive and experimental groups ( < 0.05), and no significant differences on the clotting time between the positive and the experimental groups ( > 0.05). In phase two, all blood samples mixed with EDTA did not clot, while the negative control had an average clotting time of 2.01 min. Blood mixed with 0.2 ml of extract had the longest coagulation time (15.49 min). Simple linear regression revealed a positive significant correlation (multiple R = 0.9450, R = 0.8931, = 0.02) implying dose-dependent anticoagulant potential. The study showed that extract may have a potential anticoagulant property due to its component SP.
抗凝剂已被用于多种循环系统疾病和血栓性疾病的治疗，以及血液学分析的采血过程中。具有抗凝特性的硫酸化多糖（SP）存在于大多数海藻中，包括[具体海藻种类]。
本研究总体旨在评估[具体海藻名称]的潜在抗凝特性。
将新鲜的[具体海藻名称]全株用蒸馏水彻底冲洗，手工挤压以获得提取物。[具体海藻名称]提取物分两个阶段进行测试。第一阶段使用9只雄性白化兔，随机且等分为三组：（1）阴性对照组（口服蒸馏水，随后将提取的血液与生理盐水混合），（2）阳性对照组（口服阿司匹林，随后将提取的血液与生理盐水混合），以及（3）实验组（口服蒸馏水，随后将提取的血液与[具体海藻名称]提取物混合）。通过玻片法和试管法测量凝血时间来评估血液凝固情况。在第二阶段，使用三只明显健康的成年犬的外周血。采血进行三次。每次采血时，样本分为五等份：（1）阴性对照（生理盐水溶液），（2）阳性对照（乙二胺四乙酸[EDTA]），以及（3 - 5）实验组处理，分别加入0.1、0.15和0.2 ml的[具体海藻名称]提取物。通过试管法测量凝血时间来评估凝血情况。
第一阶段结果显示，阴性对照组与阳性对照组和实验组之间的凝血时间存在显著差异（P < 0.05），而阳性对照组与实验组之间的凝血时间无显著差异（P > 0.05）。在第二阶段，所有与EDTA混合的血液样本均未凝固，而阴性对照组的平均凝血时间为2.01分钟。与0.2 ml[具体海藻名称]提取物混合的血液凝血时间最长（15.49分钟）。简单线性回归显示存在显著正相关（复相关系数R = 0.9450，相关系数R = 0.8931，P = 0.02），这意味着具有剂量依赖性抗凝潜力。该研究表明，[具体海藻名称]提取物因其所含的SP成分可能具有潜在的抗凝特性。
