Laboratory of Molecular Immunopharmacology, Key Laboratory of Animal Models and Human Disease Mechanisms, National Kunming High Level Biosafety Research Center for Nonhuman Primates, Kunming Institute of Zoology, Chinese Academy of Sciences, Kunming, Yunnan, China.
Department of Pharmacology, University of Health Sciences, Lahore, Pakistan.
J Med Virol. 2022 Jun;94(6):2669-2674. doi: 10.1002/jmv.27648. Epub 2022 Feb 25.
Human immunodeficiency virus (HIV) infection is fast becoming widespread in the world with 37.7 million people living with HIV in 2020. Antiretroviral therapy involving chemical drugs has declined acquired immunodeficiency syndrome (AIDS)-related mortality and improved the life quality of AIDS/HIV sufferers. However, the emergence of drug resistance and side effects are the main obstacles for the long-term use of these chemicals as antiretroviral therapy. Recently, a lot of emphasis is being put on finding naturally occurring drug candidates that show activity against HIV and can be potentially used as antiretroviral therapy. In this study, different medicinal plants, Pistacia khinjuk, Teucrium stocksianum, Uncaria tomentosa, Pistacia integerrima, Trigonella gharuensis, and Artocarpus lakoocha, were explored for their anti-HIV potential. Syncytium and p24 assays were performed to determine antiviral activity, while the MTT assay was used to determine cytotoxicity. Results showed that extracts from all six plants inhibited HIV replication in vitro. Also, extracts from Pistacia khinjuk, Teucrium stocksianum, Uncaria tomentosa, and Pistacia integerrima showed low cytotoxicity with a 50% cytotoxicity concentration value of >200 μM. Results of this study indicate that there is potential in these natural extracts to become candidate drugs to be used as complementary and alternative medicine for HIV infection.
人类免疫缺陷病毒（HIV）感染在全球范围内迅速蔓延，2020 年全球有 3770 万人感染 HIV。抗逆转录病毒治疗涉及化学药物，降低了艾滋病相关死亡率，提高了艾滋病/艾滋病毒感染者的生活质量。然而，耐药性和副作用的出现是这些化学物质作为抗逆转录病毒治疗长期使用的主要障碍。最近，人们非常重视寻找具有抗 HIV 活性的天然药物候选物，这些候选物可能被用作抗逆转录病毒治疗。在这项研究中，研究了不同的药用植物，包括黄连木、筋骨草、钩藤、野核桃、黑种草和印度榕，以评估它们的抗 HIV 潜力。通过合胞体和 p24 测定来评估抗病毒活性，同时通过 MTT 测定来评估细胞毒性。结果表明，来自这六种植物的提取物都能抑制 HIV 在体外的复制。此外，黄连木、筋骨草、钩藤和野核桃的提取物显示出低细胞毒性，其 50%细胞毒性浓度值大于 200 μM。这项研究的结果表明，这些天然提取物具有成为候选药物的潜力，可作为 HIV 感染的补充和替代药物。
