analysis of carotenoid biosynthesis pathway in cassava ( Crantz).

The apocarotenoids play a vital role in plant growth and development process, especially strigolactones, which can induce rooting and help in the interaction with symbiotic microbes in plants. They also act as colorants, antioxidants, hormones, signalling components, scent/aroma constituents and chromophores. approaches are valuable in reducing the complexity regarding gene networks in plants that help to develop new biotechnological and bioinformatics tactics in crop improvement programmes. An comparative genomic analysis of the key enzymes encoding genes involved in apocarotenoid biosynthesis in cassava was carried out using template plants such as arabidopsis, tomato, potato and sweet potato. Forty carotenoid genes were identified, and the nucleotide sequences were subjected to various regulatory sequence analyses such as transcription factor prediction, CpG island analysis, microRNA regulatory analysis and promotor sequence analysis. The corresponding protein sequences were subjected to domain/motif analysis and phylogenetic analysis. The expression profile of apocarotenoid genes in cassava were generated and subcellular localization prediction was done to identify the distribution of the proteins. The results indicated that the apocarotenoid protein domains were conserved in template plants and cassava. Eighteen transcription factors like MYB, BBR-BPC, bHLH and NAC were associated with the identified carotenoid genes in cassava. The apocarotenoid genes were found to be expressed in all the major parts of the plants. These genes were distributed in 17 of 18 cassava chromosomes and the third one contained maximum number of genes. MiRNA regulatory analysis identified three microRNAs, namely miR159a, miR171b and miR396a which were significantly associated with carotenoid biosynthesis in cassava and the pathway was reconstructed by incorporating the above information. A better understanding of the genes and pathway associated with carotenoid biosynthesis in cassava would be helpful in the breeding programme to develop improved carotenoid rich varieties.