Wicomb W N, Novitzky D, Cooper D K, Rose A G
J Surg Res. 1986 Mar;40(3):276-84. doi: 10.1016/0022-4804(86)90161-7.
A system has been developed for the continuous hypothermic perfusion of isolated hearts using a clear fluid perfusate. Myocardial viability has been maintained after periods of storage of up to 48 hr. Pig hearts stored in this way showed almost normal hemodynamic performance on subsequent functional testing. Orthotopic allotransplantation or autotransplantation of baboon hearts stored for 48 hr was followed by good immediate and long-term function. Baboons receiving allotransplants survived until rejection. Three of four of those autotransplanted survived until electively sacrificed at 1, 3, and 12 months; all showed normal hemodynamic function on cardiac catheterization and normal myocardial histology.
已经开发出一种使用透明液体灌注液对离体心脏进行持续低温灌注的系统。在长达48小时的储存期后，心肌活力得以维持。以这种方式储存的猪心脏在随后的功能测试中显示出几乎正常的血液动力学性能。对储存48小时的狒狒心脏进行原位同种异体移植或自体移植后，即刻和长期功能良好。接受同种异体移植的狒狒存活至发生排斥反应。四只自体移植的狒狒中有三只存活至分别在1个月、3个月和12个月时被选择性处死；所有动物在心脏导管检查中均显示正常的血液动力学功能，心肌组织学正常。
