Nitrate Is an Environmental Cue in the Gut for Salmonella enterica Serovar Typhimurium Biofilm Dispersal through Curli Repression and Flagellum Activation via Cyclic-di-GMP Signaling.

Curli, a major component of the bacterial biofilms in the intestinal tract, activates pattern recognition receptors and triggers joint inflammation after infection with Salmonella enterica serovar Typhimurium. The factors that allow Typhimurium to disperse from biofilms and invade the epithelium to establish a successful infection during acute inflammation remain unknown. Here, we studied Typhimurium biofilms and to understand how the inflammatory environment regulates the switch between multicellular and motile Typhimurium in the gut. We discovered that nitrate generated by the host is an environmental cue that induces Typhimurium to disperse from the biofilm. Nitrate represses production of an important biofilm component, curli, and activates flagella via the modulation of intracellular cyclic-di-GMP levels. We conclude that nitrate plays a central role in pathogen fitness by regulating the sessile-to-motile lifestyle switch during infection. Recent studies provided important insight into our understanding of the role of c-di-GMP signaling and the regulation of enteric biofilms. Despite an improved understanding of how c-di-GMP signaling regulates Typhimurium biofilms, the processes that affect the intracellular c-di-GMP levels and the formation of multicellular communities during infections remain unknown. Here, we show that nitrate generated in the intestinal lumen during infection with Typhimurium is an important regulator of biofilm formation .