M. Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty, Republic of Kazakhstan.
National Center for Biotechnology, Nur-Sultan, Republic of Kazakhstan.
Ann Hum Biol. 2022 Feb;49(1):87-89. doi: 10.1080/03014460.2022.2039292. Epub 2022 Mar 7.
Previous studies of the genetic polymorphism of the Y-chromosome of Kazakhs were focussed on the Eastern, Central, Southern, and Western regions of Kazakhstan. In addition, many of these studies were limited to 17 Y-STR loci from the Yfiler.
To enrich the existing Kazakhstan Y-chromosome Haplotype Reference Database from the Northern Kazakh population data by a wide set of 27 Y-STR and investigate the population genetic relationships with previously published data.
Twenty-seven Y-STR loci from the Yfiler Plus PCR Amplification Kit were analysed in 382 healthy unrelated Kazakh males from Northern Kazakhstan. Genetic polymorphism was analysed using Arlequin software.
A total of 326 distinct haplotypes of the 27 Y-STR loci were observed in 382 individuals. The discrimination capacity (0.9982) and haplotype diversity (0.8534) were computed. A total of 168 alleles at single-copy loci were observed and their frequencies ranged from 0.003-0.843. The pairwise genetic distance (R) showed that the Northern Kazakh population is genetically distinct from the Chinese Kazakh population.
Genetic polymorphism shows that the potential value of 27 Y-STR loci for forensic casework in the Northern Kazakh population and the current findings might be beneficial for paternal lineages in the study of population genetics.
先前对哈萨克族 Y 染色体遗传多态性的研究集中在哈萨克斯坦的东部、中部、南部和西部地区。此外，这些研究中的许多研究仅局限于来自 Yfiler 的 17 个 Y-STR 基因座。
通过广泛的 27 个 Y-STR 位点，丰富现有的来自哈萨克北部人群的数据的哈萨克 Y 染色体单体型参考数据库，并结合已发表的数据调查其与群体遗传关系。
在来自哈萨克北部的 382 名健康无关的男性中，分析了来自 Yfiler Plus PCR 扩增试剂盒的 27 个 Y-STR 基因座。使用 Arlequin 软件分析遗传多态性。
在 382 名个体中观察到 27 个 Y-STR 基因座的 326 个独特单体型。计算了鉴别能力（0.9982）和单体型多样性（0.8534）。观察到单拷贝基因座的总共 168 个等位基因，其频率范围为 0.003-0.843。成对遗传距离（R）表明，哈萨克北部人群在遗传上与中国哈萨克人群不同。
遗传多态性表明，27 个 Y-STR 基因座对哈萨克北部人群法医学案例工作具有潜在价值，并且目前的发现可能对研究群体遗传学中的父系血统有益。
