M. Aitkhozhin Institute of Molecular Biology and Biochemistry, Almaty, Republic of Kazakhstan.
National Center for Biotechnology, Astana, Republic of Kazakhstan.
Ann Hum Biol. 2023 Feb;50(1):48-51. doi: 10.1080/03014460.2023.2170465.
The establishment of a national haplotype database is important for forensic and genetic applications and requires studying genetic polymorphisms at Y-STR sites. However, the genetic structure of the Eastern Kazakhstan population is poorly characterised.
To investigate the genetic polymorphisms of 27 Y-STR loci in the Kazakh population from Eastern Kazakhstan and analyse the population genetic relationships of the Eastern Kazakhs with other populations.
The Yfiler Plus kit was utilised to genotype 246 healthy, unrelated males from Eastern Kazakhstan. Based on the raw data, haplotype and allele frequencies along with forensic parameters were calculated, and an MDS plot was constructed.
A total of 207 haplotypes were detected, of which 186 were unique. The haplotype diversity and discrimination capacity were 0.997 and 0.841, respectively. Population comparisons showed that Eastern Kazakhs have close genetic relationships with Kazakhs from Xinjiang, China. At the same time, a difference was found between the studied population and the previous one in the same part of Kazakhstan.
The obtained haplotypes will help to expand the Kazakhstan Y-chromosome reference database and will be useful for future genetic research and forensic applications.
建立全国单体型数据库对于法医和遗传应用非常重要，需要研究 Y-STR 位点的遗传多态性。然而，哈萨克斯坦东部人群的遗传结构尚未得到充分描述。
研究哈萨克斯坦东部哈萨克人群中 27 个 Y-STR 位点的遗传多态性，并分析东部哈萨克人与其他人群的群体遗传关系。
使用 Yfiler Plus 试剂盒对来自哈萨克斯坦东部的 246 名健康、无关男性进行基因分型。根据原始数据计算单倍型和等位基因频率以及法医参数，并构建 MDS 图。
共检测到 207 个单倍型，其中 186 个为独特单倍型。单倍型多样性和鉴别能力分别为 0.997 和 0.841。群体比较表明，东部哈萨克人与中国新疆的哈萨克人具有密切的遗传关系。同时，与同一地区的先前研究相比，发现研究人群与之前的研究存在差异。
获得的单倍型将有助于扩展哈萨克斯坦 Y 染色体参考数据库，并将有助于未来的遗传研究和法医学应用。
