Meier J
Toxicon. 1986;24(1):41-6. doi: 10.1016/0041-0101(86)90164-9.
Individual and age-dependent variations in the venoms of 23 specimens of Bothrops atrox were studied using SDS-polyacrylamide gel electrophoresis, isoelectric focusing and different enzymic activity measurements. The electrophoretic patterns showed a decrease in total protein band numbers and a shifting from components with higher to those with lower molecular weights with increasing age of the snakes. The venoms of individuals younger than 12 months showed isoelectric focusing patterns within a pH range of 3.5-6.0, whereas those of older specimens extended from pH 3.5 to 7.0. Individual variations were found in fibrinogen-clotting and fibrinolytic activities. Despite the fact that important quantitative differences were also found in amidolytic activities measured on five chromogenic proteinase substrates, all the venoms showed the same activity profile. Specimens of the oldest age group (8 years) showed absence of some enzymatic activities.
使用SDS-聚丙烯酰胺凝胶电泳、等电聚焦和不同的酶活性测量方法，研究了23条矛头蝮蛇毒液中的个体差异和年龄依赖性差异。电泳图谱显示，随着蛇年龄的增长，总蛋白条带数量减少，且从高分子量成分向低分子量成分转变。12个月以下个体的毒液在pH值3.5 - 6.0范围内显示等电聚焦图谱，而年龄较大个体的毒液等电聚焦图谱范围从pH值3.5延伸至7.0。在纤维蛋白原凝血和纤维蛋白溶解活性方面发现了个体差异。尽管在五种发色蛋白酶底物上测得的酰胺水解活性也存在重要的定量差异，但所有毒液都显示出相同的活性谱。最年长年龄组（8岁）的样本显示出某些酶活性缺失。
