Yin Dafei, Wang Youli, Wang Liqun, Wu Yuqin, Bian Xiaoyi, Aggrey Samuel E, Yuan Jianmin
College of Animal Husbandry and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, China.
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, 100193, Beijing, China.
J Anim Sci Biotechnol. 2022 Feb 9;13(1):26. doi: 10.1186/s40104-021-00656-1.
The use of newly harvested corn in feed causes wet droppings in broilers and increased feed cost which was termed as "new season grain problem". The present study was conducted to evaluate the proteomic profile of newly harvested corn and the subsequent influence on intestinal microbiol community for broiler chickens.
Newly harvested corn stored for either half a month (HM) or two months (TM) was used, and the pasting properties, total soluble sugars, and proteomic analysis technology was used to explore the influence of storage on natural aging corn properties. Additionally, seventy-two 7-day-old Ross 308 male broiler chicken were fed with different stored corn. Apparent metabolizable energy (AME), digesta viscosity, intestinal morphology and microbiota were examined to explore the influence of feed corn storage on broiler chickens.
Pasting properties in the TM corn exhibited decreased viscoelastic properties. Proteomic studies found a total of 26 proteins that were differentially expressed between the two treatment groups. Proteins involved in starch and polysaccharides biosynthesis were upregulated in TM compared with HM. Chickens fed on TM diet had higher relative energy utilization compared to the HM birds. With increased corn storage, the relative digesta viscosity decreased significantly (P ≤ 0.05). The total number of goblet cells and lymphocytes was lower in chickens fed the TM diet. The microbiota data showed that the TM chickens had decreased abundance of diarrheal bacteria such as Hungatella hathewayi and Bacteroides fragilis, and increased butyrate-producing bacteria such as Alistipes compared to the HM chickens.
Storage of newly harvested corn induced the synthetic reaction of large molecules and changed the solubility of starch and protein with increasing soluble sugars and decreasing pasting properties that may improve the fermentation of intestinal microbiota, improve the energy utilization and protect gut health without the risk of diarrhea.
在饲料中使用新收获的玉米会导致肉鸡粪便变湿且饲料成本增加，这被称为“新季谷物问题”。本研究旨在评估新收获玉米的蛋白质组学特征及其对肉鸡肠道微生物群落的后续影响。
使用储存半个月（HM）或两个月（TM）的新收获玉米，采用糊化特性、总可溶性糖和蛋白质组分析技术来探究储存对天然老化玉米特性的影响。此外，用不同储存时间的玉米喂养72只7日龄的罗斯308雄性肉鸡。检测表观代谢能（AME）、食糜粘度、肠道形态和微生物群，以探究饲料玉米储存对肉鸡的影响。
TM玉米的糊化特性表现出粘弹性降低。蛋白质组学研究发现，两个处理组之间共有26种蛋白质差异表达。与HM相比，参与淀粉和多糖生物合成的蛋白质在TM中上调。与HM组鸡相比，饲喂TM日粮的鸡相对能量利用率更高。随着玉米储存时间增加，相对食糜粘度显著降低（P≤0.05）。饲喂TM日粮的鸡杯状细胞和淋巴细胞总数较低。微生物群数据显示，与HM组鸡相比TM组鸡中腹泻细菌如哈氏洪氏菌和脆弱拟杆菌的丰度降低，而产丁酸细菌如阿利斯杆菌的丰度增加。
新收获玉米的储存诱导了大分子的合成反应，并改变了淀粉和蛋白质的溶解性，可溶性糖增加而糊化特性降低，这可能改善肠道微生物群的发酵，提高能量利用率并保护肠道健康而无腹泻风险。
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