Ascenzi J M, Ezzell R J, Wendt T M
Appl Environ Microbiol. 1986 Jan;51(1):91-4. doi: 10.1128/aem.51.1.91-94.1986.
Revision of the official test method for the determination of the tuberculocidal activity of disinfectants is being undertaken. The current procedure lacks precision and accuracy and is not quantitative. Variability associated with carriers and the lack of temperature control were evaluated in this paper. The use of porcelain versus stainless steel carriers was also evaluated. When carriers of either type were contaminated with Mycobacterium bovis BCG, the number of organisms on the carriers varied by as much as 1.0 on the log10 scale. The average number of organisms attached to each porcelain carried was 1.10 x 10(5) CFU (range, 2.7 x 10(4) to 2.7 x 10(5) CFU), whereas the average number of organisms attached to each stainless steel carrier was 1.38 x 10(5) CFU (range, 2.9 x 10(4) to 4.0 x 10(5) CFU). The average number of cells attached to the carrier was directly proportional to the number of cells in the contaminating cell suspension. Variations in drying time did not alter the number of cells attached to the carrier. When porcelain carriers were placed in a test solution, the average number of organisms washed from the carriers was 55% of the total, with a range of 19 to 80%, whereas for stainless steel carriers, the average number was 82% of the total, with a range of 52 to 96%. Data for B. subtilis spores were similar to those for M. bovis BCG, suggesting that there may be similar problems with the Association of Official Analytical Chemists sporicidal test, which uses carriers. It was also found that the lack of an exacting temperature control could influence the outcome of the test. Changes in temperature as little as 1 degree C could influence the rate of killing of M. bovis BCG.
正在对消毒剂杀结核活性测定的官方测试方法进行修订。当前的程序缺乏精密度和准确性，且不是定量的。本文评估了与载体相关的变异性以及缺乏温度控制的情况。还评估了使用瓷质载体与不锈钢载体的情况。当任何一种类型的载体被牛分枝杆菌卡介苗污染时，载体上的微生物数量在对数 10 尺度上变化高达 1.0。附着在每个瓷质载体上的微生物平均数量为 1.10×10⁵CFU（范围为 2.7×10⁴至 2.7×10⁵CFU），而附着在每个不锈钢载体上的微生物平均数量为 1.38×10⁵CFU（范围为 2.9×10⁴至 4.0×10⁵CFU）。附着在载体上的细胞平均数量与污染细胞悬液中的细胞数量成正比。干燥时间的变化并未改变附着在载体上的细胞数量。当将瓷质载体置于测试溶液中时，从载体上洗下的微生物平均数量为总数的 55%，范围为 19%至 80%，而对于不锈钢载体，平均数量为总数的 82%，范围为 52%至 96%。枯草芽孢杆菌孢子的数据与牛分枝杆菌卡介苗的数据相似，这表明使用载体的美国官方分析化学家协会的杀孢子试验可能存在类似问题。还发现缺乏严格的温度控制可能会影响测试结果。温度仅变化 1℃就可能影响牛分枝杆菌卡介苗的杀灭速率。
