Liu Qiuchen, Liu Chang, Cai Songtao, He Song, Zhao Liancheng, Zeng Xianshun, Gong Jin
Tianjin Key Laboratory for Photoelectric Materials and Devices, School of Materials Science & Engineering, Tianjin University of Technology, Tianjin, 300384, P. R. China.
School of Chemical Engineering and Technology, Tianjin University, Tianjin, 300072, P. R. China.
Dalton Trans. 2022 Feb 22;51(8):3116-3121. doi: 10.1039/d1dt03900h.
Considering the scarcity of palladium ion probes with subcellular organelle targeting, especially probes with near-infrared (NIR) emission wavelength fluorophores, our group has been working to overcome this problem and looking forward to providing potential practical tools for exploring the toxicity of palladium ions at the subcellular level. In this paper, a novel colorimetric and NIR fluorescent probe, BHCy-Pd, for the specific detection of palladium ions (Pd) in lysosomes an internal charge-transfer (ICT) mechanism was designed and synthesized. As expected, BHCy-Pd exhibited a rapid, selective, and sensitive response for palladium with an ultralow limit of detection at 5.9 nM, accompanied by a distinct color change from purple to blue. Furthermore, BHCy-Pd can be made into a simple test strip for rapid and easy detection of Pd in practical applications. Importantly, BHCy-Pd is capable of specific distribution in lysosomes, and thus can detect Pd in real-time, thereby providing a potential tool for studying the cytotoxicity of Pd ions at the subcellular level.
考虑到具有亚细胞器靶向功能的钯离子探针稀缺，尤其是具有近红外（NIR）发射波长荧光团的探针，我们团队一直致力于克服这一问题，并期望为在亚细胞水平探索钯离子的毒性提供潜在的实用工具。本文设计并合成了一种用于特异性检测溶酶体中钯离子（Pd）的新型比色和近红外荧光探针BHCy-Pd，其基于内电荷转移（ICT）机制。正如预期的那样，BHCy-Pd对钯表现出快速、选择性和灵敏的响应，检测限低至5.9 nM，同时伴有从紫色到蓝色的明显颜色变化。此外，BHCy-Pd可制成简单的测试条，便于在实际应用中快速检测Pd。重要的是，BHCy-Pd能够特异性分布于溶酶体中，从而可实时检测Pd，为在亚细胞水平研究Pd离子的细胞毒性提供了潜在工具。
