Universidade de São Paulo, Faculdade de Medicina, Hospital das Clínicas, LIM-06 - Imunopatologia da Esquistossomose, São Paulo, São Paulo, Brazil.
Universidade de São Paulo, Faculdade de Medicina, Hospital das Clínicas, São Paulo, São Paulo, Brazil.
Rev Inst Med Trop Sao Paulo. 2022 Feb 2;64:e9. doi: 10.1590/S1678-9946202264009. eCollection 2022.
The Western-blotting technique was applied to identify antigenic fractions of excretory-secretory Toxocara canis antigen recognized by IgG antibodies throughout an experimental infection in mice challenged by different inocula. Mice were inoculated with 5, 50 and 500 embryonated eggs and serum samples were collected 15, 30, 60, 90 and 120 days post-infection. Serum samples were analyzed using an excretory-secretory Toxocara antigen. Antibodies recognized antigenic fractions from 30 to 90 kDa. The protein fraction of 30-35 kDa was the most frequently recognized regardless of the size of inoculum and the stage of infection represented by the different collection times, but the antigenic recognition was more evident in groups infected with 50 and 500 eggs. This study presents an antigenic panel of the excretory-secretory antigen of T. canis and suggests that the 30-35 kDa antigenic fraction is a promising marker of the infection and should be further explored in future studies on experimental toxocariasis.
Western-blotting 技术被应用于鉴定排泄-分泌抗原中被 IgG 抗体识别的抗原片段，这些抗原片段在感染实验中被不同接种物挑战的小鼠中产生。小鼠被接种 5、50 和 500 个卵囊，在感染后 15、30、60、90 和 120 天采集血清样本。使用排泄-分泌的 Toxocara 抗原分析血清样本。抗体识别 30 至 90 kDa 的抗原片段。无论接种物的大小和不同采集时间所代表的感染阶段如何，30-35 kDa 的蛋白质片段都是最常被识别的，但在感染 50 和 500 个卵囊的组中，抗原识别更为明显。本研究提出了 T. canis 排泄-分泌抗原的抗原谱，并表明 30-35 kDa 的抗原片段是感染的有前途的标志物，应在未来的实验性旋毛虫病研究中进一步探索。
