Fundação Oswaldo Cruz-Fiocruz, Instituto Carlos Chagas, Laboratório de Pesquisa em Apicomplexa, Curitiba, PR, Brasil.
Universidade Estadual de Campinas, Instituto de Biologia, Departamento de Genética, Evolução, Microbiologia e Imunologia, Campinas, SP, Brasil.
Mem Inst Oswaldo Cruz. 2022 Feb 4;116:e210227. doi: 10.1590/0074-02760210227. eCollection 2022.
Plasmodium vivax, the major cause of malaria in Latin America, has a large subtelomeric multigene family called vir. In the P. vivax genome, about 20% of its sequences are vir genes. Vir antigens are grouped in subfamilies according to their sequence similarities and have been shown to have distinct roles and subcellular locations. However, little is known about vir subfamilies, especially when comes to their functions.
To evaluate the diversity, antigenicity, and adhesiveness of Plasmodium vivax VIR-E.
Vir-E genes were amplified from six P. vivax isolates from Manaus, North of Brazil. The presence of naturally acquired antibodies to recombinant PvBrVIR-E and PvAMA-1 was evaluated by ELISA. Binding capacity of recombinant PvBrVIR-E was assessed by adhesion assay to CHO-ICAM1 cells.
Despite vir-E sequence diversity, among those identified sequences, a representative one was chosen to be expressed as recombinant protein. The presence of IgM or IgG antibodies to PvBrVIR-E was detected in 23.75% of the study population while the presence of IgG antibodies to PvAMA-1 antigen was 66.25% in the same population. PvBrVIR-E was adhesive to CHO-ICAM1.
PvBrVIR-E was antigenic and adhesive to CHO-ICAM1.
引起拉丁美洲疟疾的主要病原体间日疟原虫（Plasmodium vivax）拥有一个大型的端粒下多基因家族，称为 vir。在 P. vivax 基因组中，约 20%的序列为 vir 基因。根据序列相似性，vir 抗原被分为亚家族，并且具有不同的作用和亚细胞定位。然而，人们对 vir 亚家族知之甚少，特别是在功能方面。
评估间日疟原虫 VIR-E 的多样性、抗原性和黏附性。
从巴西北部玛瑙斯的 6 株 P. vivax 分离株中扩增 Vir-E 基因。通过 ELISA 评估天然获得的针对重组 PvBrVIR-E 和 PvAMA-1 的抗体。通过黏附试验评估重组 PvBrVIR-E 与 CHO-ICAM1 细胞的结合能力。
尽管存在 vir-E 序列多样性，但在鉴定出的序列中，选择了一个有代表性的序列进行表达为重组蛋白。在研究人群中，有 23.75%的人存在针对 PvBrVIR-E 的 IgM 或 IgG 抗体，而同一人群中针对 PvAMA-1 抗原的 IgG 抗体存在率为 66.25%。PvBrVIR-E 可黏附 CHO-ICAM1。
PvBrVIR-E 具有抗原性，可黏附 CHO-ICAM1。
