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所有线粒体翻译产物都是在膜结合核糖体上合成的吗?

Are all mitochondrial translation products synthesized on membrane-bound ribosomes?

作者信息

Marzuki S, Hibbs A R

出版信息

Biochim Biophys Acta. 1986 Mar 26;866(2-3):120-4. doi: 10.1016/0167-4781(86)90108-9.

DOI:10.1016/0167-4781(86)90108-9
PMID:3513839
Abstract

The Arrhenius kinetics for the synthesis of var1, which is a hydrophilic protein of the mitochondrial ribosomes, have been compared to that of other mitochondrial translation products, which are hydrophobic subunits of the respiratory enzyme complexes. Our results indicate that, in the yeast Saccharomyces cerevisiae, the hydrophilic var1 protein is synthesized on membrane-associated mitochondrial ribosomes which cannot be distinguished from those responsible for the synthesis of the hydrophobic mitochondrial translation products.

摘要

已将线粒体核糖体的亲水性蛋白质var1合成的阿累尼乌斯动力学与呼吸酶复合物的疏水性亚基等其他线粒体翻译产物的动力学进行了比较。我们的结果表明,在酿酒酵母中,亲水性var1蛋白是在与膜相关的线粒体核糖体上合成的,这些核糖体与负责合成疏水性线粒体翻译产物的核糖体无法区分。

相似文献

1
Are all mitochondrial translation products synthesized on membrane-bound ribosomes?所有线粒体翻译产物都是在膜结合核糖体上合成的吗?
Biochim Biophys Acta. 1986 Mar 26;866(2-3):120-4. doi: 10.1016/0167-4781(86)90108-9.
2
Assembly of the mitochondrial ribosomes in a temperature-conditional mutant of Saccharomyces cerevisiae defective in the synthesis of the var1 protein.在酿酒酵母的一个温度条件突变体中,var1蛋白合成缺陷导致线粒体核糖体的组装。
Biochim Biophys Acta. 1987 Feb 27;908(2):179-87. doi: 10.1016/0167-4781(87)90057-1.
3
Biogenesis of mitochondria: a temperature sensitivity mutation affecting the mitochondrially synthesized var1 protein of Saccharomyces cerevisiae.线粒体的生物发生:一个影响酿酒酵母线粒体合成的var1蛋白的温度敏感性突变。
Arch Biochem Biophys. 1980 Aug;203(1):260-70. doi: 10.1016/0003-9861(80)90176-9.
4
Evidence that synthesis of the Saccharomyces cerevisiae mitochondrially encoded ribosomal protein Var1p may be membrane localized.有证据表明酿酒酵母线粒体编码的核糖体蛋白Var1p的合成可能定位于膜上。
Eukaryot Cell. 2003 Jun;2(3):651-3. doi: 10.1128/EC.2.3.651-653.2003.
5
Incorporation of the yeast mitochondrial ribosomal protein Mrp2 into ribosomal subunits requires the mitochondrially encoded Var1 protein.酵母线粒体核糖体蛋白Mrp2整合到核糖体亚基中需要线粒体编码的Var1蛋白。
Mol Gen Genet. 1995 May 10;247(3):379-86. doi: 10.1007/BF00293206.
6
Trans action and the var1 determinant region on yeast mitochondrial DNA. Specific labeling of mitochondrial translation products in zygotes.酵母线粒体DNA上的转座作用和var1决定区。合子中线粒体翻译产物的特异性标记。
J Biol Chem. 1981 Jun 25;256(12):6496-501.
7
Molecular genetics of the peptidyl transferase center and the unusual Var1 protein in yeast mitochondrial ribosomes.酵母线粒体核糖体中肽基转移酶中心和异常Var1蛋白的分子遗传学
Experientia. 1996 Dec 15;52(12):1148-57. doi: 10.1007/BF01952114.
8
The association of var1 with the 38 S mitochondrial ribosomal subunit in yeast.酵母中var1与38S线粒体核糖体亚基的关联。
J Biol Chem. 1979 Dec 25;254(24):12653-61.
9
Mitochondrially-encoded protein Var1 promotes loss of respiratory function in Saccharomyces cerevisiae under stressful conditions.线粒体编码蛋白 Var1 在应激条件下促进酿酒酵母呼吸功能丧失。
Eur J Cell Biol. 2013 Apr-May;92(4-5):169-74. doi: 10.1016/j.ejcb.2013.02.001. Epub 2013 Mar 13.
10
Proteolytic breakdown of membrane-associated polypeptides in mitochondria of Saccharomyces cerevisiae.酿酒酵母线粒体中膜相关多肽的蛋白水解降解
Methods Enzymol. 1995;260:495-503. doi: 10.1016/0076-6879(95)60161-9.

引用本文的文献

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Molecular crowding inhibits U-insertion/deletion RNA editing in vitro: consequences for the in vivo reaction.分子拥挤在体外抑制U插入/缺失RNA编辑:对体内反应的影响
PLoS One. 2013 Dec 23;8(12):e83796. doi: 10.1371/journal.pone.0083796. eCollection 2013.
2
Mitochondrial protein synthesis, import, and assembly.线粒体蛋白质合成、输入和组装。
Genetics. 2012 Dec;192(4):1203-34. doi: 10.1534/genetics.112.141267.
3
The yeast GTPase Mtg2p is required for mitochondrial translation and partially suppresses an rRNA methyltransferase mutant, mrm2.
酵母GTP酶Mtg2p是线粒体翻译所必需的,并且部分抑制rRNA甲基转移酶突变体mrm2。
Mol Biol Cell. 2005 Feb;16(2):954-63. doi: 10.1091/mbc.e04-07-0622. Epub 2004 Dec 9.
4
Evidence that synthesis of the Saccharomyces cerevisiae mitochondrially encoded ribosomal protein Var1p may be membrane localized.有证据表明酿酒酵母线粒体编码的核糖体蛋白Var1p的合成可能定位于膜上。
Eukaryot Cell. 2003 Jun;2(3):651-3. doi: 10.1128/EC.2.3.651-653.2003.
5
Multiple interactions involving the amino-terminal domain of yeast mtRNA polymerase determine the efficiency of mitochondrial protein synthesis.涉及酵母线粒体RNA聚合酶氨基末端结构域的多种相互作用决定了线粒体蛋白质合成的效率。
J Biol Chem. 2003 May 16;278(20):18695-701. doi: 10.1074/jbc.M301399200. Epub 2003 Mar 10.
6
A cytoplasmic male sterility-associated mitochondrial peptide in common bean is post-translationally regulated.菜豆中一种与细胞质雄性不育相关的线粒体肽受到翻译后调控。
Plant Cell. 1998 Jul;10(7):1217-28. doi: 10.1105/tpc.10.7.1217.
7
Mitochondrial ribosomal proteins (MRPs) of yeast.酵母的线粒体核糖体蛋白(MRPs)
Biochem J. 1998 Feb 1;329 ( Pt 3)(Pt 3):433-48. doi: 10.1042/bj3290433.