利用基于 CRISPR 的活细胞成像技术揭示 ecDNA 的行为。
Shedding Light on ecDNA Behavior Using CRISPR-Based Live-Cell Imaging.
机构信息
Department of Pediatric Oncology/Hematology, Charité-Universitätsmedizin Berlin, Berlin, Germany.
Berlin Institute of Health, Berlin, Germany.
出版信息
Cancer Discov. 2022 Feb;12(2):293-295. doi: 10.1158/2159-8290.CD-21-1525.
Extrachromosomal oncogene amplification on extrachromosomal DNA (ecDNA) has emerged as a hallmark of many cancers. In this issue, Yi and colleagues developed a CRISPR-based method for imaging ecDNA in live cells, termed ecTag. Using ecTag, the authors reveal important features of ecDNA in cancer cells such as their random mitotic segregation and clustering into transcriptionally active hubs after mitosis..
细胞外染色体癌基因扩增是许多癌症的一个标志。在本期杂志中，Yi 及其同事开发了一种基于 CRISPR 的活细胞中外源 DNA 成像方法，称为 ecTag。利用 ecTag，作者揭示了癌细胞中外源 DNA 的一些重要特征，如它们在有丝分裂过程中的随机分离，以及有丝分裂后聚集到转录活跃的中心。
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