• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

康百年洗液通过抑制耐氟康唑菌株生长及激活Dectin-1信号通路改善小鼠外阴阴道念珠菌病

Kangbainian Lotion Ameliorates Vulvovaginal Candidiasis in Mice by Inhibiting the Growth of Fluconazole-Resistant and the Dectin-1 Signaling Pathway Activation.

作者信息

Chen Zewei, Luo Tengshuo, Huang Fengke, Yang Fuzhen, Luo Wenting, Chen Guanfeng, Cao Mengfei, Wang Fengyun, Zhang Jun

机构信息

School of Pharmaceutical Sciences, Guangzhou University of Chinese Medicine, Guangzhou, China.

School of Chemistry and Molecular Engineering, East China Normal University, Shanghai, China.

出版信息

Front Pharmacol. 2022 Jan 20;12:816290. doi: 10.3389/fphar.2021.816290. eCollection 2021.

DOI:10.3389/fphar.2021.816290
PMID:35140608
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8819624/
Abstract

Vulvovaginal candidiasis (VVC) is an infectious disease caused by species, which affects millions of women worldwide every year. The resistance to available antifungal drugs for clinical treatment is a growing problem. The treatment of refractory VVC caused by azole-resistant is still facing challenges. However, research on new antifungal drugs is progressing slowly. Although a lot of reports on new antifungal drugs, only three new antifungal drugs (Isavuconazole, ibrexafungerp, and rezafungin) and two new formulations of posaconazole were marketed over the last decade. Chinese botanical medicine has advantages in the treatment of drug-resistant VVC, such as outstanding curative effects and low adverse reactions, which can improve patients' comfort and adherence to therapy. Kangbainian lotion (KBN), a Chinese botanical formulation, has achieved very good clinical effects in the treatment of VVC. In this study, we investigated the antifungal and anti-inflammatory effects of KBN at different doses in fluconazole-resistant (FLC-resistant) VVC model mice. We further studied the antifungal mechanism of KBN against FLC-resistant () and the anti-inflammatory mechanism correlated with the Dectin-1 signaling pathway. and results showed that KBN had strong antifungal and anti-inflammatory effects in FLC-resistant VVC, such as inhibiting the growth of and vaginal inflammation. Further studies showed that KBN inhibited the biofilm and hypha formation, reduced adhesion, inhibited ergosterol synthesis and the expression of ergosterol synthesis-related genes , and reduced the expression of drug-resistant efflux pump genes and of FLC-resistant . In addition, results showed that KBN reduced the expression of inflammatory factor proteins TNF-α, IL-1β, and IL-6 in vaginal tissues, and inhibited the expression of proteins related to the Dectin-1 signaling pathway. In conclusion, our study revealed that KBN could ameliorate vaginal inflammation in VVC mice caused by FLC-resistance . This effect may be related to inhibiting the growth of FLC-resistance and Dectin-1 signaling pathway activation.

摘要

外阴阴道假丝酵母菌病（VVC）是一种由特定菌种引起的传染病，每年影响着全球数百万女性。临床治疗中对现有抗真菌药物的耐药性是一个日益严重的问题。由耐唑类假丝酵母菌引起的难治性VVC的治疗仍面临挑战。然而，新型抗真菌药物的研究进展缓慢。尽管有很多关于新型抗真菌药物的报道，但在过去十年中，只有三种新型抗真菌药物（艾沙康唑、ibrexafungerp和瑞扎芬净）以及泊沙康唑的两种新剂型上市。中药在治疗耐药性VVC方面具有优势，如疗效显著且不良反应低，可提高患者的舒适度和治疗依从性。康百年洗液（KBN）是一种中药制剂，在治疗VVC方面取得了很好的临床效果。在本研究中，我们研究了不同剂量的KBN在氟康唑耐药（FLC耐药）VVC模型小鼠中的抗真菌和抗炎作用。我们进一步研究了KBN对FLC耐药白色念珠菌（白色念珠菌）的抗真菌机制以及与Dectin-1信号通路相关的抗炎机制。体内和体外结果表明，KBN在FLC耐药VVC中具有强大的抗真菌和抗炎作用，如抑制白色念珠菌生长和阴道炎症。进一步研究表明，KBN抑制生物膜和菌丝形成，减少黏附，抑制麦角固醇合成以及麦角固醇合成相关基因ERG11和ERG3的表达，并降低FLC耐药白色念珠菌耐药外排泵基因CDR1和MDR1的表达。此外，体内结果表明，KBN降低了阴道组织中炎性因子蛋白TNF-α、IL-1β和IL-6的表达，并抑制了与Dectin-1信号通路相关的蛋白表达。总之，我们的研究表明，KBN可以改善由FLC耐药白色念珠菌引起的VVC小鼠的阴道炎症。这种作用可能与抑制FLC耐药白色念珠菌生长和激活Dectin-1信号通路有关。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ce2/8819624/60aa3be4efa1/fphar-12-816290-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ce2/8819624/300ca77a25b2/fphar-12-816290-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ce2/8819624/6306920124de/fphar-12-816290-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ce2/8819624/bdda1e77030c/fphar-12-816290-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ce2/8819624/33a3ddf719c5/fphar-12-816290-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ce2/8819624/22bab323af90/fphar-12-816290-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ce2/8819624/59a5f2a54534/fphar-12-816290-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ce2/8819624/19e64e2d71f7/fphar-12-816290-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ce2/8819624/56bdda219f11/fphar-12-816290-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ce2/8819624/0de31c612560/fphar-12-816290-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ce2/8819624/60aa3be4efa1/fphar-12-816290-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ce2/8819624/300ca77a25b2/fphar-12-816290-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ce2/8819624/6306920124de/fphar-12-816290-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ce2/8819624/bdda1e77030c/fphar-12-816290-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ce2/8819624/33a3ddf719c5/fphar-12-816290-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ce2/8819624/22bab323af90/fphar-12-816290-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ce2/8819624/59a5f2a54534/fphar-12-816290-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ce2/8819624/19e64e2d71f7/fphar-12-816290-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ce2/8819624/56bdda219f11/fphar-12-816290-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ce2/8819624/0de31c612560/fphar-12-816290-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/1ce2/8819624/60aa3be4efa1/fphar-12-816290-g010.jpg

相似文献

1
Kangbainian Lotion Ameliorates Vulvovaginal Candidiasis in Mice by Inhibiting the Growth of Fluconazole-Resistant and the Dectin-1 Signaling Pathway Activation.康百年洗液通过抑制耐氟康唑菌株生长及激活Dectin-1信号通路改善小鼠外阴阴道念珠菌病
Front Pharmacol. 2022 Jan 20;12:816290. doi: 10.3389/fphar.2021.816290. eCollection 2021.
2
The liquid Kangfuxin (KFX) has efficient antifungal activity and can be used in the treatment of vulvovaginal candidiasis in mice.康复新液（KFX）具有高效抗真菌活性，可用于治疗小鼠外阴阴道念珠菌病。
Lett Appl Microbiol. 2022 Apr;74(4):564-576. doi: 10.1111/lam.13641. Epub 2022 Feb 8.
3
[Pulsatilla decoction inhibits vulvovaginal Candida albicans proliferation and reduces inflammatory cytokine levels in vulvovaginal candidiasis mice].白头翁汤抑制外阴阴道念珠菌病小鼠模型中白色念珠菌的增殖并降低炎性细胞因子水平
Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi. 2016 Feb;32(2):153-7.
4
Quercetin Assists Fluconazole to Inhibit Biofilm Formations of Fluconazole-Resistant Candida Albicans in In Vitro and In Vivo Antifungal Managements of Vulvovaginal Candidiasis.槲皮素辅助氟康唑在体外抑制耐氟康唑白色念珠菌生物膜形成及外阴阴道念珠菌病的体内抗真菌治疗中发挥作用。
Cell Physiol Biochem. 2016;40(3-4):727-742. doi: 10.1159/000453134. Epub 2016 Nov 30.
5
Prevalence of vulvovaginal candidiasis among pregnant women in the Ho municipality, Ghana: species identification and antifungal susceptibility of Candida isolates.加纳霍市孕妇外阴阴道假丝酵母菌病的流行情况：假丝酵母菌分离株的种属鉴定和抗真菌药敏试验。
BMC Pregnancy Childbirth. 2020 May 6;20(1):266. doi: 10.1186/s12884-020-02963-3.
6
In vitro outcomes of quercetin on Candida albicans planktonic and biofilm cells and in vivo effects on vulvovaginal candidiasis. Evidences of its mechanisms of action.槲皮素对白色念珠菌浮游和生物膜细胞的体外作用及其对阴道假丝酵母菌病的体内作用。其作用机制的证据。
Phytomedicine. 2023 Jun;114:154800. doi: 10.1016/j.phymed.2023.154800. Epub 2023 Apr 2.
7
Pulsatilla decoction inhibits Candida albicans proliferation and adhesion in a mouse model of vulvovaginal candidiasis via the Dectin-1 signaling pathway.白头翁汤通过 Dectin-1 信号通路抑制白色念珠菌在阴道念珠菌病小鼠模型中的增殖和黏附。
J Ethnopharmacol. 2018 Sep 15;223:51-62. doi: 10.1016/j.jep.2018.05.018. Epub 2018 May 26.
8
Vulvovaginal candidiasis: species distribution, fluconazole resistance and drug efflux pump gene overexpression.外阴阴道念珠菌病：菌种分布、氟康唑耐药性及药物外排泵基因过表达
Mycoses. 2014 Oct;57(10):584-91. doi: 10.1111/myc.12204.
9
[INVESTIGATION ON ANTIFUNGAL SUSCEPTIBILITY OF CANDIDA YEASTS IN PREGNANT PATIENTS WITH CONFIRMED VULVOVAGINAL CANDIDIASIS AND THEIR NEWBORNS.].[确诊外阴阴道念珠菌病的孕妇及其新生儿念珠菌酵母菌的抗真菌药敏性调查。]
Akush Ginekol (Sofiia). 2016;55(4):20-29.
10
Mrr2 mutations and upregulation are associated with increased fluconazole resistance in Candida albicans isolates from patients with vulvovaginal candidiasis.Mrr2 突变和上调与外阴阴道念珠菌病患者分离的白念珠菌中氟康唑耐药性增加有关。
Lett Appl Microbiol. 2020 Feb;70(2):95-101. doi: 10.1111/lam.13248. Epub 2019 Dec 5.

引用本文的文献

1
Impact of Clove Oil on Biofilm Formation in and Its Effects on Mice with Candida Vaginitis.丁香油对白色念珠菌性阴道炎生物膜形成的影响及其对小鼠的作用
Mycobiology. 2025 Aug 7;53(5):661-675. doi: 10.1080/12298093.2025.2537477. eCollection 2025.
2
and synergistic inhibition of targeting cell membranes by and extracts.以及[具体物质1]和[具体物质2]提取物对靶细胞膜的协同抑制作用。 （注：原文中“and”前应该有具体物质名称未给出，这里进行了补充说明以使译文更完整）
Front Microbiol. 2024 Sep 11;15:1456240. doi: 10.3389/fmicb.2024.1456240. eCollection 2024.
3
Synergistic activity and mechanism of KBN lotion and miconazole nitrate against drug-resistant biofilms.

本文引用的文献

1
Recurrent Vulvovaginal Candidiasis: a Dynamic Interkingdom Biofilm Disease of and .复发性外阴阴道念珠菌病：一种涉及[具体内容缺失]的动态跨界生物膜疾病
mSystems. 2021 Aug 31;6(4):e0062221. doi: 10.1128/mSystems.00622-21. Epub 2021 Aug 10.
2
Guideline: Vulvovaginal candidosis (AWMF 015/072, level S2k).指南：外阴阴道假丝酵母菌病（AWMF 015/072，S2k 级别）。
Mycoses. 2021 Jun;64(6):583-602. doi: 10.1111/myc.13248. Epub 2021 Feb 27.
3
Detection of ERG11 Overexpression in Candida albicans isolates from environmental sources and clinical isolates treated with inhibitory and subinhibitory concentrations of fluconazole.
KBN 洗液与硝酸咪康唑联合抗耐药生物膜活性及机制研究。
Front Cell Infect Microbiol. 2024 Aug 29;14:1426791. doi: 10.3389/fcimb.2024.1426791. eCollection 2024.
4
Licochalcone A Protects Vaginal Epithelial Cells Against Candida albicans Infection Via the TLR4/NF-κB Signaling Pathway.甘草查尔酮 A 通过 TLR4/NF-κB 信号通路保护阴道上皮细胞免受白念珠菌感染。
J Microbiol. 2024 Jul;62(7):525-533. doi: 10.1007/s12275-024-00134-z. Epub 2024 May 31.
检测环境来源的白色念珠菌分离株和氟康唑抑制和亚抑菌浓度处理的临床分离株中 ERG11 的过表达。
Mycoses. 2021 Feb;64(2):220-227. doi: 10.1111/myc.13208. Epub 2020 Dec 11.
4
Synergistic Effect of Berberine Hydrochloride and Fluconazole Against Resistant Isolates.盐酸小檗碱与氟康唑对耐药菌株的协同作用
Front Microbiol. 2020 Jul 2;11:1498. doi: 10.3389/fmicb.2020.01498. eCollection 2020.
5
(Thunb.) Hyl. Extracts from Different Plant Parts: Phenolic Composition, Antioxidant, and Anti-Inflammatory Activities.(Thunb.) Hyl. 不同植物部位提取物：酚类成分、抗氧化和抗炎活性。
Molecules. 2020 Mar 5;25(5):1153. doi: 10.3390/molecules25051153.
6
Analgesic and anti-inflammatory effects and mechanism of action of borneol on photodynamic therapy of acne.龙脑对光动力疗法治疗痤疮的镇痛抗炎作用及作用机制。
Environ Toxicol Pharmacol. 2020 Apr;75:103329. doi: 10.1016/j.etap.2020.103329. Epub 2020 Jan 8.
7
Synergistic Effects and Mechanisms of Combined Treatment With Harmine Hydrochloride and Azoles for Resistant .盐酸哈尔明与唑类联合治疗耐药菌的协同作用及机制
Front Microbiol. 2019 Oct 15;10:2295. doi: 10.3389/fmicb.2019.02295. eCollection 2019.
8
Antifungal drugs: What brings the future?抗真菌药物：未来的发展方向是什么？
Med Mycol. 2019 Jun 1;57(Supplement_3):S328-S343. doi: 10.1093/mmy/myz012.
9
Zerumbone inhibits biofilm formation and hyphal growth.姜烯酮抑制生物膜形成和菌丝生长。
Can J Microbiol. 2019 Oct;65(10):713-721. doi: 10.1139/cjm-2019-0155. Epub 2019 Jun 3.
10
Effect of Shikonin Against Biofilms.紫草素对生物膜的作用。
Front Microbiol. 2019 May 14;10:1085. doi: 10.3389/fmicb.2019.01085. eCollection 2019.