丁香油对白色念珠菌性阴道炎生物膜形成的影响及其对小鼠的作用
Impact of Clove Oil on Biofilm Formation in and Its Effects on Mice with Candida Vaginitis.
作者信息
Gao Yuan, Wang Zhuo, Wang Xin, Xu Yan, Luo Jianghan, Li Siming, Yang Yang, Yan Lijun, Zou Xiang
机构信息
School of Pharmacy, Harbin University of Commerce, Harbin, Heilongjiang, PR China.
Engineering Research Center of Natural Antineoplastic Drugs, Ministry of Education, Harbin University of Commerce, Harbin, Heilongjiang, PR China.
出版信息
Mycobiology. 2025 Aug 7;53(5):661-675. doi: 10.1080/12298093.2025.2537477. eCollection 2025.
In this study, the effects of Clove oil (CEO) on biofilm formation of () were investigated using XTT and crystal violet staining methods. The therapeutic efficacy of CEO was studied using ICR mouse model with candida vaginitis. The potential targets and pathways of the CEO's effect against were predicted by network pharmacology. The relative gene levels of CDC42, Ste11, Hst7, CEK1, and CPH1 were evaluated by qPCR. Subsequently, the protein levels of CDC42, CEK1, and p-CEK1 were determined by Western blot. The results indicated CEO can inhibit adhesion, mycelium formation and biofilm maturation process. By regulating the related genes and proteins, the CEO can inhibit biofilm maturation process, and weaken the ability of to cause disease. For candida vaginitis ICR mouse model, CEO can lower inflammatory cytokines levels, alleviated the symptoms of vaginitis, and play a good therapeutic effect.
在本研究中，使用XTT和结晶紫染色方法研究了丁香油（CEO）对（）生物膜形成的影响。使用患有念珠菌性阴道炎的ICR小鼠模型研究了CEO的治疗效果。通过网络药理学预测了CEO抗（）作用的潜在靶点和途径。通过qPCR评估CDC42、Ste11、Hst7、CEK1和CPH1的相对基因水平。随后，通过蛋白质印迹法测定CDC42、CEK1和p-CEK1的蛋白质水平。结果表明，CEO可以抑制（）的黏附、菌丝形成和生物膜成熟过程。通过调节相关基因和蛋白质，CEO可以抑制（）生物膜成熟过程，并削弱（）致病能力。对于念珠菌性阴道炎ICR小鼠模型，CEO可以降低炎性细胞因子水平，减轻阴道炎症状，并发挥良好的治疗效果。
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