Dastsooz Hassan, Alizadeh Ahad, Habibzadeh Parham, Nariman Ali, Hosseini Asieh, Mansoori Yaser, Haghi-Aminjan Hamed
Department of Life Sciences and Systems Biology, University of Turin, Turin, Italy.
Candiolo, C/o IRCCS, IIGM-Italian Institute for Genomic Medicine, Turin, Italy.
Front Genet. 2022 Jan 24;12:791919. doi: 10.3389/fgene.2021.791919. eCollection 2021.
Gastrointestinal (GI) cancers are responsible for approximately half of cancer-related deaths, highlighting the need for the identification of distinct and common features in their clinicopathological characteristics. Long ncRNA (lncRNAs), which are involved in competitive endogenous RNA (ceRNA) networks with critical roles in biological processes, constitute a substantial number of non-coding RNAs. Therefore, our study aimed to investigate the similarities and differences in the ceRNA networks of The Cancer Genome Atlas (TCGA)-GI cancers. We performed a comprehensive bioinformatics analysis of ceRNA networks for TCGA-GI cancers in terms of the deferential mRNA, lncRNA, and miRNA expression levels, ceRNA networks, overall survival analysis, correlation analysis, pathological cancer stages, and gene set enrichment analysis. Our study revealed several common and distinct mRNAs and lncRNAs with prognostic values in these networks. It was specifically noteworthy that lncRNA was found to be shared in almost all GI cancers. Moreover, the most common shared mRNAs between GI cancers were and For each cancer ceRNA network, we found that the expression level of a number of lncRNAs and mRNAs was specific. Furthermore, our study provided compelling evidence that several genes, most notably , can act as novel proto-oncogenes in cancers. This, in turn, can highlight their role as new prognostic and therapeutic targets. Moreover, we found cell cycle and extracellular matrix structural constituent as the top shared KEGG and molecular function, respectively, among GI cancers. Our study revealed several known lncRNAs and known and unknown mRNAs in GI cancers with diagnostic and prognostic values.
胃肠道（GI）癌症约占癌症相关死亡人数的一半，这凸显了识别其临床病理特征中独特和共同特征的必要性。长链非编码RNA（lncRNAs）参与竞争性内源性RNA（ceRNA）网络，在生物过程中起关键作用，构成了大量的非编码RNA。因此，我们的研究旨在调查癌症基因组图谱（TCGA）-GI癌症的ceRNA网络中的异同。我们对TCGA-GI癌症的ceRNA网络进行了全面的生物信息学分析，内容涉及差异mRNA、lncRNA和miRNA表达水平、ceRNA网络、总生存分析、相关性分析、病理癌症分期和基因集富集分析。我们的研究揭示了这些网络中几种具有预后价值的常见和独特的mRNA和lncRNA。特别值得注意的是，lncRNA在几乎所有GI癌症中都有共享。此外，GI癌症之间最常见的共享mRNA是 和 对于每个癌症ceRNA网络，我们发现许多lncRNA和mRNA的表达水平是特异性的。此外，我们的研究提供了令人信服的证据，表明几个基因，最显著的是 ，可以在癌症中作为新的原癌基因发挥作用。这反过来又可以突出它们作为新的预后和治疗靶点的作用。此外，我们发现细胞周期和细胞外基质结构成分分别是GI癌症中最常见的共享KEGG通路和分子功能。我们的研究揭示了GI癌症中几种具有诊断和预后价值的已知lncRNA以及已知和未知的mRNA。
