King S J, Miller H R, Woodbury R G, Newlands G F
Eur J Immunol. 1986 Feb;16(2):151-5. doi: 10.1002/eji.1830160208.
The distribution of the predominant chymotrypsin-like enzyme of mucosal mast cells (rat mast cell protease II: RMCP II) was examined in naive and Nippostrongylus-primed rats both before and after the induction of systemic anaphylaxis. Anaphylactic secretion of RMCP II following i.v. challenge of primed rats with worm antigen was accompanied by significant depletion of this enzyme from the jejunal and gastric mucosae; the concentrations were not altered in the ileum and colon. Despite significant increases in the levels of RMCP II in lung and mesenteric lymph node following infection with N. brasiliensis there was no anaphylactic depletion of this enzyme from these sites. No RMCP II was detected in liver, spleen, kidney or bone marrow either before or after systemic anaphylaxis. Mucosal mast cells were depleted from the jejunal, gastric and colonic mucosae following antigen challenge of primed rats. These data provide further evidence that gastrointestinal mucosal mast cells are the major source of secreted RMCP II following systemic anaphylaxis in the rat.
在未致敏和经巴西日圆线虫致敏的大鼠中，于全身过敏反应诱导前后，对黏膜肥大细胞的主要类胰凝乳蛋白酶（大鼠肥大细胞蛋白酶II：RMCP II）的分布进行了检查。用蠕虫抗原静脉内攻击致敏大鼠后，RMCP II的过敏反应性分泌伴随着该酶从小肠和胃黏膜的显著耗竭；回肠和结肠中的浓度未改变。尽管感染巴西日圆线虫后肺和肠系膜淋巴结中RMCP II的水平显著升高，但这些部位并未出现该酶的过敏反应性耗竭。在全身过敏反应前后，肝脏、脾脏、肾脏或骨髓中均未检测到RMCP II。用抗原攻击致敏大鼠后，空肠、胃和结肠黏膜中的黏膜肥大细胞减少。这些数据进一步证明，在大鼠全身过敏反应后，胃肠道黏膜肥大细胞是分泌的RMCP II的主要来源。
