Paddock S W, Albrecht-Buehler G
Exp Cell Res. 1986 Apr;163(2):525-38. doi: 10.1016/0014-4827(86)90083-2.
Cytoskeletal aspects of monensin-treated 3T3 cells with rotating nuclei were studied by immunofluorescence. The pattern of intermediate filaments and microtubules appeared unchanged when compared with control cells having a stationary nucleus. In contrast, the actin microfilament bundles appeared to have a consistent distribution in cells with rotating nuclei. Typically, we did not find long microfilament bundles that traverse the length of the cytoplasm of cells that were fixed at the time of nuclear rotation. Instead, there was a local distribution of short microfilament bundles situated ventrally to the nucleus and oriented at various angles to one another and to the predominant distribution of microfilament bundles in the cell. The observations suggest that the actin cytoskeleton is reorganized locally before or during rotation of the nucleus.
通过免疫荧光研究了莫能菌素处理的具有旋转细胞核的3T3细胞的细胞骨架特征。与具有静止细胞核的对照细胞相比，中间丝和微管的模式未发生变化。相反，肌动蛋白微丝束在具有旋转细胞核的细胞中似乎具有一致的分布。通常，我们在细胞核旋转时固定的细胞中未发现横穿细胞质全长的长微丝束。取而代之的是，短微丝束在细胞核腹侧呈局部分布，它们彼此之间以及与细胞中微丝束的主要分布方向呈不同角度排列。这些观察结果表明，肌动蛋白细胞骨架在细胞核旋转之前或期间在局部进行了重组。
