Faculty of Veterinary Medicine, Research Unit in Epidemiology and Risk Analysis Applied to Veterinary Sciences (UREAR-ULiège), Fundamental and Applied Research for Animals and Health (FARAH) Center, University of Liège, Liège, Belgium; School of Animal and Veterinary Sciences, University of Adelaide, Adelaide, SA, Australia.
School of Animal and Veterinary Sciences, University of Adelaide, Adelaide, SA, Australia.
Vet Microbiol. 2022 Mar;266:109360. doi: 10.1016/j.vetmic.2022.109360. Epub 2022 Jan 31.
In 2013, Brucella melitensis biovar 1 was recovered from the stomach contents of a scimitar-horned Oryx - SHO (Oryx dammah) aborted foetus, and from the articular fluid of a sand gazelle (Gazella marica) in a captive wildlife collection near Abu Dhabi, United Arab Emirates. Other evidence of exposure to the pathogen was collected through serological testing (Rose Bengal test) and B. melitensis-specific PCR of samples from captive wildlife kept in six different enclosures. A Multiple Locus Variable Number of Tandem Repeats (VNTR) Analysis (MLVA) using 15 markers showed that the two strains isolated in animals kept in enclosures, located 1300 m apart from each other, shared an identical genotype. The phylogenetic analysis of MLVA-15 profiles retrieved from the public database suggested that these strains belong to the African clade, clustering regionally in the UAE, Oman and Qatar. This is the first confirmed case of B. melitensis in a SHO, an African antelope extinct in the wild and warrants further investigation.
2013 年，从阿联酋阿布扎比附近的一个圈养野生动物收藏中，一只镰刀角大羚羊（Oryx dammah）流产胎儿的胃内容物和一只沙瞪羚（Gazella marica）的关节液中分离出了 1 型布鲁氏菌。通过对圈养野生动物的血清学检测（虎红平板凝集试验）和布鲁氏菌特异性 PCR 检测，收集了其他与病原体接触的证据。从分布在 1300 米远的六个不同围栏中的动物中采集的样本进行了 15 个标记的多位点可变数目串联重复序列分析（MLVA），结果显示，在围栏中饲养的动物中分离出的两种菌株具有相同的基因型。从公共数据库中检索到的 MLVA-15 图谱的系统发育分析表明，这些菌株属于非洲分支，在阿联酋、阿曼和卡塔尔地区存在区域性聚类。这是首例在 SHO（一种已在野外灭绝的非洲羚羊）中确认的布鲁氏菌感染病例，需要进一步调查。
