Ameni Gobena, Zewude Aboma, Bayissa Berecha, Alfaki Ibrahim Abdalla, Albizreh Abdallah A, Alhosani Naeema, Alkalbani Meera Saeed, Abdelhalim Mohamed Moustafa, Abdelazim Assem Sobhi, Koliyan Rafeek Aroul, Kayaf Kaltham, Nuaimat Mervat Mari Al, Barigye Robert, Tibbo Markos, Eltahir Yassir Mohammed
Department of Veterinary Medicine, College of Agriculture and Veterinary Medicine, United Arab Emirates University, Al Ain City, Abu Dhabi Emirate, UAE.
Vaccine Production and Drug Formulation Directorate, National Veterinary Institute, Debre Zeit, Oromia Region, Ethiopia.
Transbound Emerg Dis. 2025 Jun 10;2025:6666896. doi: 10.1155/tbed/6666896. eCollection 2025.
Small ruminants are important livestock species, which function as a major source of protein, in the United Arab Emirates (UAE), although infections such as infection can hamper their productivity. However, there is currently a paucity of epidemiological data on infections in small ruminants in the UAE. This study therefore aimed to estimate the seroprevalence of infection and evaluate the associated risk factors in 272 flocks encompassing 2730 small ruminants in the Emirate of Abu Dhabi. In addition, DNA of the was tested in seropositive small ruminant. Multispecies competitive enzyme-linked immunosorbent assay (cELISA) and multispecies indirect ELISA (iELISA) were used to detect antibodies, while real-time quantitative polymerase chain reaction (qPCR) was used to detect the DNA of genus Brucella and its major species ( (.) , , and ). Flock seroprevalences of infection were 13.6% (95% CI: 9.8%-18.3%) and 25.5% (95% CI: 20.3%-31.0%) based on iELISA and cELISA, respectively. While animal seroprevalences were 2.31% (95% CI: 1.8-2.9) and 4.84% (95% CI: 4.1-5.7) on the basis of iELISA and cELISA, respectively. Flock seroprevalence was associated with flock size, whereas animal seroprevalence was associated with region, holding type, species, and age. The genus DNA was detected in the sera of 28.21% (11/39) of seropositive small ruminants. The 11 genus positive sera were further identified into three , three mixed infections of with either or , two , and one . While the remaining two were not positive for any of the three species. In conclusion, although animal seroprevalences were low by both ELISA tests, flock seroprevalences were relatively high. Besides, was the dominant species that was detected in the sera small ruminants posing zoonotic threat to the public. Therefore, the results of this study warrant for re-enforcement of the control and preventive measures of infections in small ruminants.
小反刍动物是重要的家畜品种，在阿拉伯联合酋长国（阿联酋）是蛋白质的主要来源，尽管诸如[感染名称]感染会影响其生产力。然而，目前阿联酋小反刍动物[感染名称]感染的流行病学数据匮乏。因此，本研究旨在估计阿布扎比酋长国272个包含2730只小反刍动物的羊群中[感染名称]感染的血清阳性率，并评估相关风险因素。此外，对血清学阳性的小反刍动物检测[病原体名称]的DNA。采用多物种竞争酶联免疫吸附测定（cELISA）和多物种间接酶联免疫吸附测定（iELISA）检测[病原体名称]抗体，同时采用实时定量聚合酶链反应（qPCR）检测布鲁氏菌属及其主要物种（[物种名称1]、[物种名称2]、[物种名称3]和[物种名称4]）的DNA。基于iELISA和cELISA，羊群[感染名称]感染的血清阳性率分别为13.6%（95%可信区间：9.8%-18.3%）和25.5%（95%可信区间：20.3%-31.0%）。而基于iELISA和cELISA，动物血清阳性率分别为2.31%（95%可信区间：1.8-2.9）和4.84%（95%可信区间：4.1-5.7）。羊群血清阳性率与羊群规模有关，而动物血清阳性率与地区、饲养类型、物种和年龄有关。在28.21%（11/39）的血清学阳性小反刍动物血清中检测到[病原体名称]属DNA。11份[病原体名称]属阳性血清进一步鉴定为3份[物种名称1]、3份[物种名称1]与[物种名称2]或[物种名称3]的混合感染、2份[物种名称2]和1份[物种名称3]。而其余2份对这三个物种均为阴性。总之，尽管两种ELISA检测的动物血清阳性率较低，但羊群血清阳性率相对较高。此外，[物种名称1]是在小反刍动物血清中检测到的主要物种，对公众构成人畜共患病威胁。因此，本研究结果有必要加强对小反刍动物[感染名称]感染的控制和预防措施。
