Asgharzadeh Mohammad, Taghinejad Zahra, Mahdavipoor Behroz, Asgharzadeh Vahid, Kafil Hossein Samadi, Rashedi Jalil
Biotechnology Research Center and Faculty of Paramedicine, Tabriz University of Medical Sciences, Tabriz, Iran.
Hematology and Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.
Infez Med. 2021 Dec 10;29(4):583-588. doi: 10.53854/liim-2904-12. eCollection 2021.
Primary (MTB) infection doesn't provide protection against secondary infection and patients can suffer from multiple strains of simultaneously. The aim of this study was to use molecular genotyping to identify cases of mixed infection in Northwest of Iran. One hundred and twenty-one positive culture isolates of were prepared from patients consecutively in Northwest of Iran from March 2017 to March 2018 and then microevolution and mix infection were assessed using the mycobacterial interspersed repetitive unit-variable number tandem repeat (MIRU-VNTR) method. MIRU-VNTR analysis revealed that nine samples (7.3%) had double alleles in at least one locus, as observed by five cases of microevolution, and four cases (3.3%) of mixed infection. According to this study, mixed infection in Northwest of Iran has significantly decreased compared to 13 years ago (7.1% decreased to 3.3%), and in order to eradicate tuberculosis it is necessary to identify all cases of mixed infection, at least in recurrent cases, in the future.
原发性（结核分枝杆菌）感染不能预防继发性感染，患者可能同时感染多种菌株。本研究的目的是利用分子基因分型来识别伊朗西北部的混合感染病例。2017年3月至2018年3月期间，从伊朗西北部的患者中连续制备了121株结核分枝杆菌阳性培养分离株，然后使用分枝杆菌散布重复单位-可变数目串联重复序列（MIRU-VNTR）方法评估微进化和混合感染情况。MIRU-VNTR分析显示，9个样本（7.3%）在至少一个位点有双等位基因，5例出现微进化，4例（3.3%）为混合感染。根据本研究，与13年前相比，伊朗西北部的混合感染显著减少（从7.1%降至3.3%），为根除结核病，未来有必要识别所有混合感染病例，至少在复发病例中。
