Gene Expression Laboratory, Salk Institute for Biological Studies, 10010 North Torrey Pines, La Jolla, CA 92037, USA.
STAR Protoc. 2022 Jan 25;3(1):101130. doi: 10.1016/j.xpro.2022.101130. eCollection 2022 Mar 18.
microRNAs (miRNAs) have unique gene regulatory effects in different neuronal subpopulations. Here, we describe a protocol to identify neuronal subtype-specific effects of a miRNA in murine motor neuron subpopulations. We detail the preparation of primary mouse spinal tissue for single cell RNA sequencing and bioinformatics analyses of pseudobulk expression data. This protocol applies differential gene expression testing approaches to identify miRNA target networks in heterogeneous neuronal subpopulations that cannot otherwise be captured by bulk RNA sequencing approaches. For complete details on the use and execution of this protocol, please refer to Amin et al. (2021).
microRNAs (miRNAs) 在不同的神经元亚群中具有独特的基因调控作用。在这里，我们描述了一种鉴定 miRNA 在小鼠运动神经元亚群中特异性作用的方案。我们详细介绍了用于单细胞 RNA 测序的原代小鼠脊髓组织的制备和伪群体表达数据的生物信息学分析。该方案应用差异基因表达测试方法，鉴定了在异质神经元亚群中无法通过批量 RNA 测序方法捕获的 miRNA 靶网络。有关此方案的使用和执行的完整详细信息，请参阅 Amin 等人。（2021 年）。
