Laboratory of Biotechnology, Conservation and Valorization of Natural Resources, Faculty of Sciences DM, Sidi Mohammed Ben Abdellah University, P.O. Box 1796 Atlas, Fez, Morocco.
The Institute for Infectious Disease Research, McMaster University, 1280 Main Street West, Hamilton, ON, Canada.
Environ Sci Pollut Res Int. 2022 Jun;29(30):45589-45600. doi: 10.1007/s11356-022-19151-9. Epub 2022 Feb 11.
The medicinal plant Juniperus oxycedrus is less recognized for the diversity of its fungal endophytes and their potential to produce extracellular enzymes. The present study is the first report on the isolation and identification of a mesophilic endophytic strain JO-A, Preussia africana, from fresh stems of the J. oxycedrus endemic tree in the Ifrane region-Morocco, and the evaluation of its ability to produce cellulases. A one-time multi-parameter one-factor screening was optimized to select factors that enhance cellulase production in P. africana. The maximum production of both CMCase and FPase activities were 1.913 IU.mL and 0.885 IU.mL, respectively, when the medium was supplemented with 2% w/v glucose. These remarkable titers were tenfold greater than those obtained under the initial non-optimized conditions. This mesophilic P. africana JO-A strain grows and actively produces cellulases at 37 °C demonstrating its great potential for various biotechnology applications. The cellulolytic extract showed the highest enzymatic activities at pH 5.0 and 50 °C with a half-life of 24 h at 50 °C.
药用植物刺柏（Juniperus oxycedrus）的真菌内生菌多样性及其产生细胞外酶的潜力尚未得到充分认识。本研究首次从摩洛哥伊夫兰地区刺柏（J. oxycedrus）的新鲜茎中分离并鉴定出一种嗜温内生菌株 JO-A，即非洲侧耳菌（Preussia africana），并评估了其产生纤维素酶的能力。采用单次多参数单因素筛选法对影响 P. africana 纤维素酶产生的因素进行了优化。当培养基中添加 2%（w/v）葡萄糖时，CMCase 和 FPase 活性的最大产量分别为 1.913 IU.mL 和 0.885 IU.mL，分别是初始非优化条件下的十倍。这种嗜温的 P. africana JO-A 菌株在 37°C 下生长并能积极产生纤维素酶，这表明它在各种生物技术应用中有很大的潜力。纤维素酶提取物在 pH 值为 5.0 和 50°C 时显示出最高的酶活性，在 50°C 下半衰期为 24 小时。
