Li Xin, Liu Xin-Chan, Ding Xu, Liu Xiao-Meng, Cao Niu-Ben, Deng Yu, Hou Yu-Bo, Yu Wei-Xian
Department of Periodontology, Hospital of Stomatology, Jilin University, Jilin University Stomatology Hospital, Changchun City, China.
Department of Oral Implantology, Hospital of Stomatology, Jilin University, Jilin University Stomatology Hospital, Changchun City, China.
Oral Dis. 2023 May;29(4):1812-1825. doi: 10.1111/odi.14148. Epub 2022 Feb 19.
Periodontitis is closely associated with kidney disease and reactive oxygen species (ROS) involvement. Mitochondria are the primary source of both endogenous ROS and renal energy. We investigated whether resveratrol (RSV) prevents renal injury and mitochondrial dysfunction in periodontitis rats.
Thirty male Wistar rats were divided into control, experimental periodontitis (Ep) and Ep-RSV groups. To induce periodontitis, a steel ligature was placed on the cervix of the bilateral first maxillary molars. RSV (50 mg/kg/day) to the Ep-RSV group and vehicle to the Ep and control groups were gavaged. After 8 weeks, alveolar bone loss, pocket depth, gingival blood index and tooth mobility were assessed. Oxidative stress parameters, mitochondrial structure, mitochondrial membrane potential (MMP), mitochondrial ROS, adenosine triphosphate (ATP), sirtuin 1 (SIRT1) and peroxisome proliferator-activated receptor-γ coactivator-1α (PGC-1α) were analysed in renal. Renal function and histology were also evaluated.
Compared with the control group, the Ep group showed renal structural destruction, elevated oxidative stress levels, mitochondrial structure destruction, MMP loss, mitochondrial ROS accumulation, ATP reduction, and decreased SIRT1 and PGC-1α levels. RSV prevented these destruction (p < 0.05). However, there was no significant impairment in renal function (p > 0.05).
Periodontitis induces mitochondrial dysfunction in renal tissues. Resveratrol exerts a preventive effect on periodontitis-induced kidney injury by preventing mitochondrial dysfunction.
牙周炎与肾脏疾病及活性氧（ROS）参与密切相关。线粒体是内源性ROS和肾脏能量的主要来源。我们研究了白藜芦醇（RSV）是否能预防牙周炎大鼠的肾损伤和线粒体功能障碍。
将30只雄性Wistar大鼠分为对照组、实验性牙周炎（Ep）组和Ep-RSV组。为诱导牙周炎，在双侧上颌第一磨牙颈部放置钢丝结扎线。对Ep-RSV组给予RSV（50mg/kg/天），对Ep组和对照组给予赋形剂，经口灌胃。8周后，评估牙槽骨吸收、牙周袋深度、牙龈出血指数和牙齿松动度。分析肾脏中的氧化应激参数、线粒体结构、线粒体膜电位（MMP）、线粒体ROS、三磷酸腺苷（ATP）、沉默调节蛋白1（SIRT1）和过氧化物酶体增殖物激活受体γ共激活因子1α（PGC-1α）。还评估了肾功能和组织学。
与对照组相比，Ep组表现出肾脏结构破坏、氧化应激水平升高、线粒体结构破坏、MMP丧失、线粒体ROS积累、ATP减少以及SIRT1和PGC-1α水平降低。RSV可预防这些破坏（p<0.05）。然而，肾功能无明显损害（p>0.05）。
牙周炎可导致肾组织线粒体功能障碍。白藜芦醇通过预防线粒体功能障碍对牙周炎诱导的肾损伤发挥预防作用。
