Dental Research Division, School of Dentistry, Paulista University, São Paulo, São Paulo, Brazil.
Department of Periodontology, Faculty of Dentistry, Toronto, Ontario, Canada.
J Periodontal Res. 2019 Jun;54(3):225-232. doi: 10.1111/jre.12622. Epub 2018 Oct 22.
This study aimed at investigating the effect of the systemic administration of resveratrol (RESV) on oxidative stress during experimental periodontitis in rats subjected to cigarette smoke inhalation.
Experimental periodontitis (EP) was induced in 26 male Wistar rats by the insertion of a ligature around one of the first mandibular and maxillary molars. The animals were assigned randomly to the following groups: cigarette smoke inhalation (CSI; 3 times/d, 8 minutes/d) + resveratrol (10 mg/Kg), that is, SMK + RESV (n = 13) and cigarette smoke inhalation + placebo, that is, SMK + PLAC (n = 13). The substances were administered daily for 30 days (19 days prior and 11 days following EP induction), and then, the animals were euthanized. The maxillary specimens were processed for morphometric analysis of bone loss, and the tissue surrounding the first maxillary molars was collected for mRNA quantification of Sirtuin 1 (SIRT1) by real-time PCR. The gingival tissues surrounding the mandibular first molars were collected for quantification of superoxide dismutase 1 (SOD1) and nicotinamide adenine dinucleotide phosphatase oxidase (NADPH) using an ELISA assay.
Reduced bone loss was demonstrated in animals in the SMK + RESV group as compared to those in the SMK + PLAC (P < 0.05) group on the basis of morphometric analysis. Resveratrol promoted higher levels of SIRT and SOD (P < 0.05) as well as reduced levels of NADPH oxidase (P < 0.05) were found in tissues derived from animals in the SMK + RESV group when compared to those in the SMK + PLAC group.
Resveratrol is an efficient therapeutic agent that reduces exacerbation of bone loss found in animals with EP that were also exposed to smoke. The results suggest that its effects could be mediated, at least in part, by its antioxidant and anti-inflammatory properties which attenuate the effects of oxidative stress on EP in the presence of cigarette smoke.
本研究旨在探讨系统性给予白藜芦醇（RESV）对吸烟诱导的实验性牙周炎大鼠氧化应激的影响。
通过结扎第一下颌和上颌磨牙之一，在 26 只雄性 Wistar 大鼠中诱导实验性牙周炎（EP）。动物随机分为以下组：吸烟（CSI；每天 3 次，每次 8 分钟）+白藜芦醇（10mg/kg），即 SMK+RESV（n=13）和吸烟+安慰剂，即 SMK+PLAC（n=13）。这些物质每天给药 30 天（EP 诱导前 19 天和后 11 天），然后处死动物。上颌标本进行骨损失的形态计量学分析，第一上颌磨牙周围的组织收集用于实时 PCR 定量 Sirtuin 1（SIRT1）的 mRNA。下颌第一磨牙周围的牙龈组织用于通过 ELISA 测定超氧化物歧化酶 1（SOD1）和烟酰胺腺嘌呤二核苷酸磷酸氧化酶（NADPH）的含量。
基于形态计量学分析，与 SMK+PLAC 组相比，SMK+RESV 组动物的骨损失减少（P<0.05）。与 SMK+PLAC 组相比，SMK+RESV 组动物组织中发现白藜芦醇促进更高水平的 SIRT 和 SOD（P<0.05），并降低 NADPH 氧化酶水平（P<0.05）。
白藜芦醇是一种有效的治疗药物，可减少同时暴露于烟雾的 EP 动物的骨损失加重。结果表明，其作用至少部分通过其抗氧化和抗炎特性介导，这些特性减轻了吸烟对 EP 中氧化应激的影响。
