Ghovvati Mahsa, Baghdasarian Sevana, Baidya Avijit, Dhal Jharana, Annabi Nasim
Department of Chemical and Biomolecular Engineering, University of California - Los Angeles, Los Angeles, California, USA.
J Biomed Mater Res B Appl Biomater. 2022 Jul;110(7):1511-1522. doi: 10.1002/jbm.b.35012. Epub 2022 Feb 11.
Injured tissues often require immediate closure to restore the normal functionality of the organ. In most cases, injuries are associated with trauma or various physical surgeries where different adhesive hydrogel materials are applied to close the wounds. However, these materials are typically toxic, have low elasticity, and lack strong adhesion especially to the wet tissues. In this study, a stretchable composite hydrogel consisting of gelatin methacrylol catechol (GelMAC) with ferric ions, and poly(ethylene glycol) diacrylate (PEGDA) was developed. The engineered material could adhere to the wet tissue surfaces through the chemical conjugation of catechol and methacrylate groups to the gelatin backbone. Moreover, the incorporation of PEGDA enhanced the elasticity of the bioadhesives. Our results showed that the physical properties and adhesion of the hydrogels could be tuned by changing the ratio of GelMAC/PEGDA. In addition, the in vitro toxicity tests confirmed the biocompatibility of the engineered bioadhesives. Finally, using an ex vivo lung incision model, we showed the potential application of the developed bioadhesives for sealing elastic tissues.
受伤组织通常需要立即闭合以恢复器官的正常功能。在大多数情况下，损伤与创伤或各种物理手术有关，在这些手术中会应用不同的粘性水凝胶材料来闭合伤口。然而，这些材料通常有毒，弹性低，尤其是对湿组织缺乏强粘附力。在本研究中，开发了一种由含三价铁离子的甲基丙烯酰化明胶邻苯二酚（GelMAC）和聚乙二醇二丙烯酸酯（PEGDA）组成的可拉伸复合水凝胶。这种工程材料可以通过邻苯二酚和甲基丙烯酸酯基团与明胶主链的化学结合而粘附在湿组织表面。此外，PEGDA的加入增强了生物粘合剂的弹性。我们的结果表明，通过改变GelMAC/PEGDA的比例可以调节水凝胶的物理性质和粘附力。此外，体外毒性试验证实了工程生物粘合剂的生物相容性。最后，使用离体肺切口模型，我们展示了所开发的生物粘合剂在密封弹性组织方面的潜在应用。
