Department of Medical Nutrition, Kyung Hee University, Yongin, Korea.
Geltech, Busan, Korea.
J Med Food. 2022 Feb;25(2):192-204. doi: 10.1089/jmf.2021.K.0124.
In this study, we investigated the protective effects of low-molecular-weight fish collagen from tilapia against melanogenesis in melanocytes, ultraviolet B (UVB)-irradiated Hs27 skin fibroblasts, and hairless mice. We observed collagen production-related pathways in UVB-irradiated Hs27 skin fibroblasts and hairless mice, and the melanogenesis-related pathways in melanocyte and UVB-irradiated hairless mice. The collagen production-related pathways were activated in the UVB-irradiated Hs27 skin fibroblasts and hairless mice. In addition, UVB exposure stimulated the melanogenesis-related pathways in melanocytes and hairless mice. However, treatment with low-molecular-weight fish collagen significantly increased the messenger RNA expressions of collagen production-related factors and significantly decreased the production of cytokines. Furthermore, treatment with low-molecular-weight fish collagen suppressed melanogenesis by inhibiting glutathione synthesis and downregulating melanocyte-inducing transcription factor expression through the suppression of cyclic AMP/protein kinase A/cAMP-responsive binding protein signaling and nitric oxide production. Low-molecular-weight fish collagen exerts protective effects against UVB-induced photoaging, through anti-inflammatory, antioxidant, and anti-melanogenesis activities and could be used for developing effective natural anti-photoaging products.
在这项研究中，我们研究了来自罗非鱼的低分子量鱼胶原蛋白对黑素细胞、紫外线 B (UVB) 照射的 Hs27 皮肤成纤维细胞和无毛小鼠的黑色素生成的保护作用。我们观察了 UVB 照射的 Hs27 皮肤成纤维细胞和无毛小鼠中的胶原蛋白生成相关途径，以及黑素细胞和 UVB 照射的无毛小鼠中的黑色素生成相关途径。UVB 照射激活了 UVB 照射的 Hs27 皮肤成纤维细胞和无毛小鼠中的胶原蛋白生成相关途径。此外，UVB 暴露刺激了黑素细胞和无毛小鼠中的黑色素生成相关途径。然而，低分子量鱼胶原蛋白的处理显著增加了胶原蛋白生成相关因子的信使 RNA 表达，并显著降低了细胞因子的产生。此外，低分子量鱼胶原蛋白通过抑制环 AMP/蛋白激酶 A/cAMP 反应结合蛋白信号和一氧化氮产生来抑制谷胱甘肽合成和下调黑素细胞诱导转录因子表达，从而抑制黑色素生成。低分子量鱼胶原蛋白通过抗炎、抗氧化和抗黑色素生成作用发挥对 UVB 诱导的光老化的保护作用，可用于开发有效的天然抗光老化产品。
