Bae In-Seon, Hoa Van-Ba, Lee Jeong-Ah, Park Won-Seo, Kim Dong-Gyun, Kim Hyoun-Wook, Seong Pil-Nam, Ham Jun-Sang
Animal Products Utilization Division, National Institute of Animal Science, RDA, Wanju 55365, Republic of Korea.
Foods. 2024 Dec 5;13(23):3934. doi: 10.3390/foods13233934.
Chronic skin conditions, such as atopic dermatitis, are characterized by a weakened skin barrier and persistent inflammation. Traditional treatments can frequently cause substantial side effects, emphasizing the need for safer alternatives. This study investigated the anti-inflammatory properties of goat meat extract and its effects on improving skin function. We conducted wound healing assays using HaCaT cells and analyzed the expression of key skin barrier-related genes. Additionally, the anti-inflammatory effects of goat meat extract were assessed in HaCaT cells stimulated with TNFα and IFNγ, as well as in LPS-treated RAW264.7 cells. Mechanistic studies focused on the activation of mitogen-activated protein kinase (MAPK) pathways. The results showed that goat meat extract significantly promoted wound closure in HaCaT cells and upregulated the expression of filaggrin, loricrin, and involucrin. The extract also reduced the production of pro-inflammatory cytokines and chemokines in both HaCaT and RAW264.7 cells. Furthermore, it inhibited the activation of the JNK, p38, and ERK pathways in TNFα/IFNγ-stimulated HaCaT cells. These findings suggest that goat meat extract improves skin barrier function and exhibits anti-inflammatory effects, indicating its potential as a therapeutic agent for chronic skin. Further research is required to investigate the in vivo effects of goat meat extract and validate its therapeutic potential.
慢性皮肤病，如特应性皮炎，其特征是皮肤屏障功能减弱和炎症持续存在。传统治疗方法常常会引发严重的副作用，这凸显了对更安全替代疗法的需求。本研究调查了羊肉提取物的抗炎特性及其对改善皮肤功能的影响。我们使用HaCaT细胞进行了伤口愈合试验，并分析了关键皮肤屏障相关基因的表达。此外，在经TNFα和IFNγ刺激的HaCaT细胞以及经LPS处理的RAW264.7细胞中评估了羊肉提取物的抗炎作用。机制研究聚焦于丝裂原活化蛋白激酶（MAPK）途径的激活。结果表明，羊肉提取物显著促进了HaCaT细胞的伤口闭合，并上调了丝聚蛋白、兜甲蛋白和内披蛋白的表达。该提取物还减少了HaCaT细胞和RAW264.7细胞中促炎细胞因子和趋化因子的产生。此外，它抑制了TNFα/IFNγ刺激的HaCaT细胞中JNK、p38和ERK途径的激活。这些发现表明，羊肉提取物可改善皮肤屏障功能并具有抗炎作用，表明其作为慢性皮肤病治疗剂的潜力。需要进一步研究来调查羊肉提取物的体内作用并验证其治疗潜力。
