转录组分析赤霉素和脱落酸在福建柏受水淹响应过程中的作用。

Transcriptome analysis of gibberellins and abscisic acid during the flooding response in Fokienia hodginsii.

机构信息

Beijing Advanced Innovation Center for Tree Breeding by Molecular Design, National Engineering Laboratory for Tree Breeding, Key Laboratory of Genetics and Breeding in Forest Trees and Ornamental Plants, Ministry of Education, College of Biological Sciences and Technology, Beijing Forestry University, Beijing, China.

Key Laboratory of Timber Forest Breeding and Cultivation for Mountainous Areas in Southern China Key Laboratory of Forest Culture and Forest Product Processing Utilization of Fujian Province, Fuzhou, China.

出版信息

PLoS One. 2022 Feb 11;17(2):e0263530. doi: 10.1371/journal.pone.0263530. eCollection 2022.

DOI:10.1371/journal.pone.0263530

PMID:35148337

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8836328/

Abstract

Flooding is one of the main abiotic stresses suffered by plants. Plants respond to flooding stress through regulating their morphological structure, endogenous hormone biosynthesis, and genetic signaling transduction. We previously found that Fokienia hodginsii varieties originating from Gutian exhibited typical flooding tolerance traits compared to three other provenances (Yongzhou, Sanming, Nanping), expressed as increased height, longer diameter at breast height (DBH), and smaller branch angle. Herein, the changes in endogenous gibberellins (GA) and abscisic acid (ABA) contents were measured under flooding stress in F. hodginsii, and ABA was found to decrease, whereas GA increased with time. Furthermore, the GA and ABA contents of the varieties originating from Gutian and the three other provenances were measured, and the results indicated that F. hodginsii from Gutian could respond more rapidly to flooding stress. The transcriptomes of the varieties originating from Gutian and the other three provenances were compared using RNA sequencing to explore the underlying genetic mechanisms of the flood-resistant phenotypes in F. hodginsii. The results indicated that two flood-stress response genes (TRINITY_DN142_c0_g2 and TRINITY_DN7657_c0_g1) were highly related to both the ABA and GA response in F. hodginsii.

摘要

洪水是植物遭受的主要非生物胁迫之一。植物通过调节其形态结构、内源激素生物合成和遗传信号转导来应对洪水胁迫。我们之前发现，来自古田的福建柏品种与其他三个产地（永州、三明、南平）相比，表现出典型的耐淹特性，表现为高度增加、胸径（DBH）更长和分枝角度更小。在此，在福建柏受到洪水胁迫下测量了内源赤霉素（GA）和脱落酸（ABA）的含量，发现 ABA 随时间减少，而 GA 增加。此外，还测量了来自古田和其他三个产地的品种的 GA 和 ABA 含量，结果表明古田的福建柏对洪水胁迫的反应更快。使用 RNA 测序比较了来自古田和其他三个产地的品种的转录组，以探讨福建柏抗洪水表型的潜在遗传机制。结果表明，两个洪水胁迫响应基因（TRINITY_DN142_c0_g2 和 TRINITY_DN7657_c0_g1）与福建柏中 ABA 和 GA 响应高度相关。