Department of Pathobiology and Immunology, Hebei University of Chinese Medicine, Shijiazhuang, Hebei, China.
J Coll Physicians Surg Pak. 2022 Mar;32(3):308-312. doi: 10.29271/jcpsp.2022.03.308.
To analyse the saliva microbial abundance and composition by 16s rRNA sequence during Helicobacter pylori (H.pylori) eradication.
Descriptive study.
Hebei University of Chinese Medicine, Hebei Provincial Hospital of Traditional Chinese Medicine, from March 2019 to January 2020.
The saliva microbial were analysed before and after the bismuth-containing quadruple therapy. A total of ten saliva samples (three groups) were enrolled in the study. The authors used the linear discriminant analysis effect size (LEfSe) method and Welch's t-test for comparative analysis to identify which taxa could be significantly affected in three groups.
H.pylori 16S rRNA gene sequence was not detected in the ten saliva samples. The abundance of Prevotella_sp._oral_clone_P4PB_83_P2 from healthy adults was higher than H.pylori-positive patients. Moreover, after the bismuth-containing quadruple therapy, the diversity and richness of saliva bacteria reduced. Lautropia, Burkholderiales, uncultured bacterium, Burkholderiaceae, and Actinomyces were enriched in H.pylori-positive patient samples after the bismuth-containing quadruple therapy.
The diversity and richness of salivary microbiome were reduced in H.pylori-positive patient, and bismuth-containing quadruple therapy affected oral microbiota. Key Words: Helicobacter pylori, Saliva, Microbiota, RNA, Bismuth.
通过 16s rRNA 序列分析幽门螺杆菌（H.pylori）根除过程中的唾液微生物丰度和组成。
描述性研究。
河北中医学院，河北省中医院，2019 年 3 月至 2020 年 1 月。
在铋四联疗法前后分析唾液微生物。本研究共纳入 10 份唾液样本（三组）。作者使用线性判别分析效应量（LEfSe）方法和 Welch 检验进行比较分析，以确定三组中哪些分类群可能受到显著影响。
在 10 份唾液样本中均未检测到 H.pylori 16S rRNA 基因序列。健康成年人唾液中普雷沃氏菌属（Prevotella_sp._oral_clone_P4PB_83_P2）的丰度高于 H.pylori 阳性患者。此外，铋四联疗法后，唾液细菌的多样性和丰富度降低。铋四联疗法后，H.pylori 阳性患者样本中富含劳特罗皮亚菌属、伯克霍尔德菌目、未培养细菌、伯克霍尔德氏菌科和放线菌属。
H.pylori 阳性患者的唾液微生物组多样性和丰富度降低，铋四联疗法影响口腔微生物群。关键词：幽门螺杆菌，唾液，微生物组，RNA，铋。
