RNA 聚合酶 III 在行动中的快照——一篇迷你综述。

Snapshots of RNA polymerase III in action - A mini review.

机构信息

State Key Laboratory of Oncogenes and Related Genes, Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, China; Shanghai Institute of Precision Medicine, Shanghai, China; Present address: Institutes of Biomedical Sciences, Fudan University, Shanghai 200032, China.

Regensburg Center for Biochemistry, University of Regensburg, 93053, Regensburg, Germany.

出版信息

Gene. 2022 May 5;821:146282. doi: 10.1016/j.gene.2022.146282. Epub 2022 Feb 8.

DOI:10.1016/j.gene.2022.146282

PMID:35149153

Abstract

RNA polymerase (Pol) III is responsible for the transcription of tRNAs, 5S rRNA, U6 snRNA, and other non-coding RNAs. Transcription factors such as TFIIIA, -B, -C, SNAPc, and Maf1 are required for promoter recognition, promoter opening, and Pol III activity regulation. Recent developments in cryo-electron microscopy and advanced purification approaches for endogenous multi-subunit complexes accelerated structural studies resulting in detailed structural insights which allowed an in-depth understanding of the molecular mechanisms underlying Pol III transcription. Here, we summarize structural data on Pol III and its regulating factors providing a three-dimensional framework to guide further analysis of RNA polymerase III.

摘要

RNA 聚合酶 (Pol) III 负责转录 tRNA、5S rRNA、U6 snRNA 和其他非编码 RNA。转录因子，如 TFIIIA、-B、-C、SNAPc 和 Maf1，对于启动子识别、启动子开放和 Pol III 活性调节是必需的。低温电子显微镜和用于内源性多亚基复合物的先进纯化方法的最新进展加速了结构研究，获得了详细的结构见解，使人们能够深入了解 Pol III 转录的分子机制。在这里，我们总结了关于 Pol III 及其调节因子的结构数据，为进一步分析 RNA 聚合酶 III 提供了一个三维框架。