Minimization and complete loss of general transcription factor proteins in the intracellular parasite .

Genome compaction is a common evolutionary feature of parasites. The unicellular, obligate intracellular parasite has one of smallest known eukaryotic genomes, and is nearly four times smaller than its distant fungi relative, the budding yeast . Comparison of the proteins encoded by compacted genomes to those encoded by larger genomes can reveal the most highly conserved features of the encoded proteins. In this study, we identified the proteins comprising the RNA polymerases and their corresponding general transcription factors by using several bioinformatic approaches to compare the transcription machinery of and . Surprisingly, our analyses revealed an overall reduction in the size of the proteins comprising transcription machinery of , which includes the loss of entire regions or functional domains from proteins, as well as the loss of entire proteins and complexes. Unexpectedly, we found that the ortholog of Rpc37 (a RNA Polymerase III subunit) more closely resembles the ortholog of Rpc37 than the ortholog of Rpc37, in both size and structure. Overall, our findings provide new insight into the minimal core eukaryotic transcription machinery and help define the most critical features of Pol components and general transcription factors.