Department of Anesthesiology, Vanderbilt University Medical Center, 1161 21(st) Avenue South, Nashville, TN 37232, USA; Department of Anesthesiology, University Medicine Greifswald, Ferdinand-Sauerbruch-Straße, 17475 Greifswald, Germany.
Department of Anesthesiology, Vanderbilt University Medical Center, 1161 21(st) Avenue South, Nashville, TN 37232, USA; Anesthesiology, TVHS VA Medical Center, 1310 24(th) Ave South, Nashville, TN 37212, USA; Department of Pharmacology, Vanderbilt University, 465 21(st) Avenue South, Nashville, TN 37232, USA.
J Pharmacol Toxicol Methods. 2022 Mar-Apr;114:107159. doi: 10.1016/j.vascn.2022.107159. Epub 2022 Feb 8.
Traumatic brain injury can lead to fatal outcomes such as disability and death. Every year, it affects many patients all over the world. Not only the primary ischemic event, but also the subsequent reperfusion can cause severe brain injury. This so-called ischemia/reperfusion injury combined with mechanical forces lead to cellular disruption. Hence, this paper describes a special in-vitro model, mimicking traumatic brain injury by combining both hypoxia/reoxygenation and compression to simulate ischemia/reperfusion injury as well as the mechanical effects that occur concurrently when suffering traumatic brain injury. Through this approach, stroke, concussion, and traumatic brain injury can be studied on different cell lines in a simplified way. We used two primary mouse brain cell cultures, namely neurons and endothelial cells. Our results show that for the different cell types, different timelines of hypoxia and compression need to be explored to achieve the optimal amount of cellular damage in order to effectively mimic traumatic brain injury. Thus, this model will be useful to test potential treatments of brain injury in future in-vitro studies.
创伤性脑损伤可导致残疾和死亡等致命后果。每年，它都会影响全世界许多患者。不仅原发性缺血事件，而且随后的再灌注也会导致严重的脑损伤。这种所谓的缺血/再灌注损伤与机械力相结合会导致细胞破裂。因此，本文描述了一种特殊的体外模型，通过结合缺氧/复氧和压缩来模拟缺血/再灌注损伤以及同时发生的机械效应来模拟创伤性脑损伤。通过这种方法，可以在简化的方式下在不同的细胞系上研究中风、脑震荡和创伤性脑损伤。我们使用了两种原代小鼠脑细胞培养物，即神经元和内皮细胞。我们的结果表明，对于不同的细胞类型，需要探索不同的缺氧和压缩时间线，以达到最佳的细胞损伤量，从而有效地模拟创伤性脑损伤。因此，该模型将有助于在未来的体外研究中测试脑损伤的潜在治疗方法。
