Medical College of Soochow University, Suzhou, 215123, Jiangsu, China.
Department of Urology, Xuzhou Central Hospital, 199 Jiefang South Road, Xuzhou, 221009, Jiangsu, China.
Clin Transl Oncol. 2022 Aug;24(8):1524-1532. doi: 10.1007/s12094-022-02791-1. Epub 2022 Feb 11.
The prolyl 3-hydroxylase family member 4 gene (P3H4) is involved in the development of human cancers. The association of P3H4 with bladder cancer (BC) prognosis is unclear. This study aimed to analyze the association of P3H4 with BC prognosis.
RNA-Seq data were downloaded from The Cancer Genome Atlas project and BC microarray datasets (GSE13507, GSE31684, and GSE32548) were downloaded from the Gene Expression Omnibus database. We analyzed the differences in P3H4 expression levels between BC tumors and non-tumor tissues and between samples with different clinical information. The association of P3H4 and P3H4-related genes with BC prognosis and the possibility of using P3H4 expression as a prognostic biomarker in BC patients were also analyzed. RevMan was used to perform the meta-analysis.
P3H4 was upregulated in BC tissues compared with the adjacent non-tumor tissues (p = 4.06e-08). Univariate Cox regression analysis and meta-analysis showed that high P3H4 expression level contributed to a poor BC prognosis (Hazard ratio, HR = 1.348, 95% CI 1.140-1.594, p = 4.89e-04; meta-analysis: HR = 1.45, 95% CI 1.10-1.91; p = 9.00e-03). Among the genes related to P3H4, the PLOD1 gene was closely associated with P3H4 expression (r = 0.620, p = 2.49e-44). Also, a meta-analysis showed that PLOD1 expression was associated with a poor prognosis in BC patients (HR = 1.77, 95% CI 1.31-2.38; p = 2.00e-04).
The P3H4 and PLOD1 genes might be used as reliable prognostic biomarkers for BC.
脯氨酰 3-羟化酶家族成员 4 基因（P3H4）参与了人类癌症的发生发展。然而，P3H4 与膀胱癌（BC）预后的关系尚不清楚。本研究旨在分析 P3H4 与 BC 预后的关系。
从癌症基因组图谱（TCGA）项目中下载 RNA-Seq 数据，并从基因表达综合数据库（GEO）中下载 BC 微阵列数据集（GSE13507、GSE31684 和 GSE32548）。我们分析了 P3H4 在 BC 肿瘤组织与非肿瘤组织之间以及不同临床信息样本之间的表达水平差异。还分析了 P3H4 及其相关基因与 BC 预后的关系，以及将 P3H4 表达作为 BC 患者预后生物标志物的可能性。RevMan 用于进行荟萃分析。
与相邻非肿瘤组织相比，BC 组织中 P3H4 表达上调（p=4.06e-08）。单因素 Cox 回归分析和荟萃分析显示，高 P3H4 表达水平与 BC 预后不良相关（风险比，HR=1.348，95%置信区间 1.140-1.594，p=4.89e-04；荟萃分析：HR=1.45，95%置信区间 1.10-1.91；p=9.00e-03）。在与 P3H4 相关的基因中，PLD1 基因与 P3H4 表达密切相关（r=0.620，p=2.49e-44）。此外，荟萃分析显示 PLD1 表达与 BC 患者预后不良相关（HR=1.77，95%置信区间 1.31-2.38；p=2.00e-04）。
P3H4 和 PLD1 基因可能作为可靠的 BC 预后生物标志物。
