Wang Dazhi, Zhang Shuyu, Chen Fufeng
1 Qingdao Municipal Hospital , Qingdao, China .
2 Cheeloo College of Medicine, Shandong University , Jinan, China .
Genet Test Mol Biomarkers. 2018 Jun;22(6):366-373. doi: 10.1089/gtmb.2018.0009. Epub 2018 May 3.
PLOD1 (procollagen-lysine, 2-oxoglutarate 5-dioxygenase 1) is important for extracellular matrix formation and is involved in various diseases, including cancer; however, its role in gastrointestinal cancer is unclear. In this study, the expression of PLOD1 in gastrointestinal carcinoma and its relationships with patient survival were examined.
Sample expression profiles were downloaded from the Gene Expression Omnibus database and methylation data were obtained from the Cancer Genome Atlas. Correlations between PLOD1 expression and clinicopathological features were analyzed by chi-square tests. Patient survival was evaluated by a Kaplan-Meier analysis.
PLOD1 expression was upregulated in gastric cancer and colorectal cancer compared with that in normal tissues. High PLOD1 levels indicated a poor prognosis. The high methylation group had a significantly lower level of PLOD1 expression.
These results indicated that PLOD1 is highly expressed in gastrointestinal carcinoma and is a potential prognostic marker and therapeutic target. The data also indicate that hypomethylation contributes to PLOD1 upregulation in gastric and colon cancers.
赖氨酰氧化酶1（PLOD1，原胶原-赖氨酸，2-氧戊二酸5-双加氧酶1）对细胞外基质形成很重要，并参与包括癌症在内的多种疾病；然而，其在胃肠道癌症中的作用尚不清楚。在本研究中，检测了PLOD1在胃肠道癌中的表达及其与患者生存的关系。
从基因表达综合数据库下载样本表达谱，并从癌症基因组图谱获得甲基化数据。通过卡方检验分析PLOD1表达与临床病理特征之间的相关性。采用Kaplan-Meier分析评估患者生存情况。
与正常组织相比，PLOD1在胃癌和结直肠癌中表达上调。PLOD1水平高表明预后不良。高甲基化组的PLOD1表达水平显著较低。
这些结果表明，PLOD1在胃肠道癌中高表达，是一种潜在的预后标志物和治疗靶点。数据还表明，低甲基化导致胃癌和结肠癌中PLOD1上调。
