Quaroni A
J Natl Cancer Inst. 1986 Apr;76(4):571-85. doi: 10.1093/jnci/76.4.571.
Twenty BALB/c mouse monoclonal antibodies to surface membrane components of confluent CaCo-2 cells were used to study the expression of small intestine markers in cultured human colon tumor cells. The antigens recognized by 15 of these antibodies were identified as membrane proteins of relatively high molecular weight, one of them corresponding to the single-chain precursor of sucrase-isomaltase. By immunofluorescence, 14 antibodies were found to stain the small intestine epithelial cells, and various distinctive patterns of antigen distribution in human jejunum were observed. Five antibodies stained exclusively the crypt cells, 3 the luminal membranes of the absorptive villus cells, and 4 the entire epithelium. In contrast, only 4 antibodies stained normal human colon tissue. The different antigens defined by this panel of monoclonal antibodies showed great variability in their expression in 14 human intestinal tumor cell lines tested. These results demonstrated a surprising similarity between many colon tumor cell lines and intestinal crypt cells and identified a set of new intestinal cell surface markers that can be used to identify and characterize intestinal tumor cells in vivo and in culture.
使用20种针对汇合的CaCo-2细胞表面膜成分的BALB/c小鼠单克隆抗体，研究培养的人结肠肿瘤细胞中小肠标志物的表达。其中15种抗体识别的抗原被鉴定为相对高分子量的膜蛋白，其中一种对应于蔗糖酶-异麦芽糖酶的单链前体。通过免疫荧光法，发现14种抗体可对小肠上皮细胞进行染色，并观察到人类空肠中各种独特的抗原分布模式。5种抗体仅对隐窝细胞染色，3种对吸收性绒毛细胞的腔面膜染色，4种对整个上皮细胞染色。相比之下，只有4种抗体可对正常人类结肠组织进行染色。这组单克隆抗体所定义的不同抗原在14种测试的人类肠道肿瘤细胞系中的表达表现出很大的变异性。这些结果表明，许多结肠肿瘤细胞系与肠道隐窝细胞之间存在惊人的相似性，并鉴定出一组新的肠道细胞表面标志物，可用于在体内和体外鉴定和表征肠道肿瘤细胞。
