Clinical Diabetes and Metabolism, Department of Medical Sciences, Uppsala University, Uppsala, Sweden.
Lab Med. 2022 Sep 1;53(5):e129-e133. doi: 10.1093/labmed/lmab126.
Research has shown that RNA extraction from adipose tissue (AT) is challenging because of high lipid content and low RNA quantity. We compared a traditional RNA extraction with a column-based method in human AT to evaluate RNA quantity and quality.
Human subcutaneous AT (n = 9) was collected through needle biopsy, and RNA was extracted using the phenol-chloroform traditional method and the RNeasy Lipid Tissue Mini Kit column-based method. The RNA quantity, quality, integrity, and expression of key AT genes were assessed.
We found that the RNA quantity and integrity were reduced by 40% and 15-20%, respectively, using the column-based method compared to the traditional method, but the findings were not statistically significant. The column-based method showed a higher 260/280 ratio (2.0) compared to the traditional method (1.8) (P <.05), suggesting lower amounts of contaminants. The expression of AT genes was comparable between methods.
The traditional extraction method provides adequate RNA yield and integrity compared to the column-based method, which is an advantage when AT specimens are small.
研究表明，由于脂肪组织（AT）中的脂质含量高和 RNA 数量低，因此从脂肪组织中提取 RNA 具有挑战性。我们比较了传统的 RNA 提取方法与基于柱的方法在人类 AT 中的应用，以评估 RNA 的数量和质量。
通过针吸活检收集人体皮下 AT（n = 9），并使用苯酚-氯仿传统方法和 RNeasy Lipid Tissue Mini Kit 基于柱的方法提取 RNA。评估 RNA 数量、质量、完整性和关键 AT 基因的表达。
与传统方法相比，基于柱的方法使 RNA 数量和完整性分别降低了 40%和 15-20%，但差异无统计学意义。与传统方法（1.8）相比，基于柱的方法显示出更高的 260/280 比值（2.0）（P<.05），提示污染物含量较低。两种方法检测 AT 基因的表达结果相当。
与基于柱的方法相比，传统的提取方法可提供足够的 RNA 产量和完整性，这在 AT 标本较小的情况下是一个优势。
