Pena Rafael Veloso, Machado Rafael Cosme, Caixeta Maxwell Batista, Araújo Paula Sampaio, de Oliveira Emília Celma, da Silva Sueli Maria, Rocha Thiago Lopes
Laboratory of Environmental Biotechnology and Ecotoxicology, Institute of Tropical Pathology and Public Health, Federal University of Goiás, Rua 235, Setor Universitário, Goiânia, Goiás CEP 74605050, Brazil.
Chemistry Institute, Federal University of Goiás, Goiânia, Goiás, Brazil.
Acta Trop. 2022 May;229:106362. doi: 10.1016/j.actatropica.2022.106362. Epub 2022 Feb 9.
Iron oxide nanoparticles (IONPs) have been indicated for the control of parasites and intermediate hosts, as well as applications in several sectors of nanomedicine. However, knowledge regarding its toxicity, mechanisms of action and the role of functionalization in gastropods that act as intermediate hosts of neglected disease parasites is still scarce. The present study aimed to evaluate the toxicity of lauric acid bilayer-functionalized IONPs (LA-IONPs), lauric acid isolated (LA) and iron ions in embryos and newly-hatched Biomphalaria glabrata. The snails were exposed to different concentrations of IONPs, LA and iron ions (1.0-97.65 mg L) during 144 h (embryos) and 96 h (newly-hatched) and multiple parameters were analyzed, such as mortality, hatching rate, developmental delay, and morphological changes. The results showed that both iron forms (LA-IONPs and iron ions) and LA promoted mortality, hatching inhibition and morphological changes in snail embryos in a concentration-dependent patterns. Embryos also showed iron bioaccumulation after exposure to both iron forms. High toxicity was observed in newly-hatched snails compared to embryos, indicating the protective role of ovigerous masses during the early developmental stages. LA induced high developmental toxicity compared to LA-IONPs and iron ions. Results showed the molluscicide activity of LA-IONPs and isolated LA, indicating their potential use as molluscicide in the snail control program.
氧化铁纳米颗粒（IONPs）已被用于控制寄生虫和中间宿主，以及在纳米医学的多个领域中应用。然而，关于其毒性、作用机制以及功能化在作为被忽视疾病寄生虫中间宿主的腹足类动物中的作用的知识仍然匮乏。本研究旨在评估月桂酸双层功能化IONPs（LA-IONPs）、分离出的月桂酸（LA）和铁离子对光滑双脐螺胚胎和刚孵化幼体的毒性。将蜗牛暴露于不同浓度的IONPs、LA和铁离子（1.0 - 97.65毫克/升）中144小时（胚胎）和96小时（刚孵化幼体），并分析多个参数，如死亡率、孵化率、发育延迟和形态变化。结果表明，两种铁形态（LA-IONPs和铁离子）以及LA均以浓度依赖性模式促进蜗牛胚胎的死亡率、孵化抑制和形态变化。胚胎在暴露于两种铁形态后也显示出铁生物积累。与胚胎相比，刚孵化的蜗牛表现出高毒性，表明卵团在早期发育阶段的保护作用。与LA-IONPs和铁离子相比，LA诱导了高发育毒性。结果显示了LA-IONPs和分离出的LA的杀螺活性，表明它们在蜗牛控制计划中作为杀螺剂的潜在用途。
