School of Life Sciences, Institute of Life Sciences and Green Development, Engineering Laboratory of Microbial Breeding and Preservation of Hebei Province, Hebei University, Baoding 071002, China; Key Laboratory of Microbial Diversity Research and Application of Hebei Province, Hebei University, Baoding 071002, China.
School of Life Sciences, Institute of Life Sciences and Green Development, Engineering Laboratory of Microbial Breeding and Preservation of Hebei Province, Hebei University, Baoding 071002, China.
Comp Biochem Physiol B Biochem Mol Biol. 2022 Apr-May;259:110719. doi: 10.1016/j.cbpb.2022.110719. Epub 2022 Feb 9.
Methyl farnesoate (MF) is an essential endocrine hormone in crustaceans, which can promote the occurrence of crustaceans molting, control morphogenesis, affect gonad development, and regulate the stress stimulation to the external environment. The farnesoic acid O-methyltransferase (FAMeT) is a key rate-limiting enzyme in MF synthesis, catalyzing the conversion of farnesoic acid (FA) into MF. Neocaridina denticulata sinensis [Decapoda] is a suitable animal model for studying crustaceans because it can reproduce many times under artificial control and has a short reproductive cycle. According to its transcriptomic and genomic information, the full-length cDNA sequence of FAMeT from N. denticulata sinensis (NdFAMeT) was cloned and the characterization of its deduced amino acid sequence was also analyzed. The relative expression of NdFAMeT in different tissues was determined. The NdFAMeT protein was recombinantly expressed in E. coli and its enzyme activity was determined. After gene knockdown by RNAi technology, the protein activity of shrimp was decreased and the individual phenotype was also observed.
甲羟呋喃酯 (MF) 是甲壳类动物中一种重要的内分泌激素，能促进甲壳类动物蜕皮，控制形态发生，影响性腺发育，并调节对外界环境的应激刺激。法呢酸 O-甲基转移酶 (FAMeT) 是 MF 合成的关键限速酶，催化法呢酸 (FA) 转化为 MF。中华锯齿米虾 [十足目] 是研究甲壳类动物的理想动物模型，因为它可以在人工控制下多次繁殖，且繁殖周期短。根据其转录组和基因组信息，克隆了中华锯齿米虾 FAMeT 的全长 cDNA 序列，并对其推导的氨基酸序列进行了特征分析。测定了 NdFAMeT 在不同组织中的相对表达。NdFAMeT 蛋白在大肠杆菌中重组表达，并测定其酶活性。通过 RNAi 技术进行基因敲低后，虾的蛋白活性降低，个体表型也发生了观察。
