Li Hongrui, Feng Dandan, Zhang Chunyu, Liu Mengfei, Wu Zixuan, Bu Yuke, Zhang Jiquan, Sun Yuying
School of Life Sciences, Hebei Basic Science Center for Biotic Interaction, Hebei University, Baoding 071002, China.
Institute of Life Science and Green Development, Hebei University, Baoding 071002, China.
Int J Mol Sci. 2024 Dec 25;26(1):65. doi: 10.3390/ijms26010065.
Farnesyl pyrophosphate synthase (FPPS) is a key enzyme in the terpenoid biosynthesis pathway, responsible for converting isopentenyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP) into farnesyl pyrophosphate (FPP). In crustaceans, FPPS plays an important role in various physiological processes, particularly in synthesizing the crustacean-specific hormone methyl farnesoate (MF). This study analyzed the evolutionary differences in the physicochemical properties, subcellular localization, gene structure, and motif composition of FPPS in (named NdFPPS) compared to other species. The significant evolutionary divergence of FPPS was observed in crustaceans, likely linked to its role in MF synthesis. After the RNA interference (RNAi)-mediated knockdown of , transcriptomic analysis of gills revealed the significant enrichment of differentially expressed genes (DEGs) in pathways related to metabolism and immunity. Gene set enrichment analysis (GSEA) showed that most of these immune-related pathways were significantly suppressed, suggesting that may indirectly regulate the immune response by modulating metabolic levels. During the early stages of infection, the expression of in the gills was significantly downregulated and subsequently returned to its original levels. Overall, our results provide new perspectives on the role of FPPS in immune regulation and enrich the functional information of FPPS.
法呢基焦磷酸合酶（FPPS）是萜类生物合成途径中的关键酶，负责将异戊烯基焦磷酸（IPP）和二甲基烯丙基焦磷酸（DMAPP）转化为法呢基焦磷酸（FPP）。在甲壳类动物中，FPPS在各种生理过程中发挥重要作用，特别是在合成甲壳类动物特有的激素法呢酸甲酯（MF）方面。本研究分析了与其他物种相比，[物种名称未给出，推测为某种甲壳类动物，暂用“（命名为NdFPPS）”表示]中FPPS在理化性质、亚细胞定位、基因结构和基序组成方面的进化差异。在甲壳类动物中观察到FPPS存在显著的进化分歧，这可能与其在MF合成中的作用有关。在RNA干扰（RNAi）介导的[物种名称未给出，推测为某种甲壳类动物，暂用“”表示]敲低后，鳃的转录组分析显示，差异表达基因（DEGs）在与代谢和免疫相关的途径中显著富集。基因集富集分析（GSEA）表明，这些与免疫相关的途径大多受到显著抑制，这表明[物种名称未给出，推测为某种甲壳类动物，暂用“”表示]可能通过调节代谢水平间接调节免疫反应。在[物种名称未给出，推测为某种甲壳类动物，暂用“”表示]感染的早期阶段，鳃中[物种名称未给出，推测为某种甲壳类动物，暂用“”表示]的表达显著下调，随后恢复到原来的水平。总体而言，我们的结果为FPPS在免疫调节中的作用提供了新的视角，并丰富了FPPS的功能信息。
