School of Life Sciences, Institute of Life Sciences and Green Development, Engineering Laboratory of Microbial Breeding and Preservation of Hebei Province, Hebei University, Baoding, 071002, China.
Xiaoshan Donghai Aquaculture Co., Ltd, Xiaoshan, 310012, China.
Fish Shellfish Immunol. 2022 Aug;127:211-218. doi: 10.1016/j.fsi.2022.06.028. Epub 2022 Jun 20.
Peroxiredoxin (Prx) is an antioxidant protein that widely exists in various organisms. To further investigate the role of Prx in the antioxidant and immune responses of Neocaridina denticulata sinensis, the full-length cDNA sequence of a Prx gene (Nd-Prx) from N. denticulata sinensis was obtained. The open reading frame (ORF) of Nd-Prx is 597 bp and encodes 198 amino acids. Amino acid similarity alignment showed that Nd-Prx contained a conserved sequence region "FYPLDFTFVCPTEI". qRT-PCR assay showed that Nd-Prx was expressed in all tested tissues and its expression was highest in the ovary. Nd-Prx was most highly expressed at 36 h after copper stimulation. Nd-Prx expression levels in hepatopancreas were significantly upregulated after Vibrio parahaemolyticus challenge (P < 0.05). In addition, the recombinant Nd-Prx was prepared and its enzyme activity was most stable at 70 °C with pH of 6.0. The antioxidant activity and DNA protection of recombinant Nd-Prx was also demonstrated. In summary, this study investigated the role of Prx in antioxidant and immune responses of N. denticulata sinensis, which might provide a foundation for further exploring Prx in immune system of crustaceans and for the application in disease control.
过氧化物酶（Prx）是一种广泛存在于各种生物中的抗氧化蛋白。为了进一步研究 Prx 在中华锯齿米虾抗氧化和免疫反应中的作用，本研究从中华锯齿米虾中获得了 Prx 基因（Nd-Prx）的全长 cDNA 序列。Nd-Prx 的开放阅读框（ORF）为 597bp，编码 198 个氨基酸。氨基酸相似性比对显示，Nd-Prx 含有一个保守序列区域“FYPLDFTFVCPTEI”。qRT-PCR 检测显示，Nd-Prx 在所有检测组织中均有表达，在卵巢中表达量最高。铜刺激 36 小时后，Nd-Prx 的表达量最高。在哈维弧菌刺激后，肝胰腺中 Nd-Prx 的表达水平显著上调（P<0.05）。此外，还制备了重组 Nd-Prx，其酶活性在 70°C 时最稳定，pH 为 6.0。还证明了重组 Nd-Prx 的抗氧化活性和 DNA 保护作用。总之，本研究调查了 Prx 在中华锯齿米虾抗氧化和免疫反应中的作用，这可能为进一步探索甲壳动物免疫系统中的 Prx 以及在疾病控制中的应用提供了基础。
