Yosri Mohammed, Elaasser Mahmoud M, Abdel-Aziz Marwa M, Ahmed Hanna Y, Amin Basma H
The Regional Center for Mycology and Biotechnology, Al-Azhar University, 11787, Nasr City, Cairo, Egypt.
Antiinflamm Antiallergy Agents Med Chem. 2022;21(1):10-23. doi: 10.2174/1871523021666220211110136.
Capparis spinosa grows in Asian and Mediterranean desert areas. Different parts of Capparis spinosa, including flowers, have been used in various folk medicine applications.
This study aims to evaluate the anti-arthritic potential of ethanolic extract of Egyptian Capparis spinosa flowers in a rat model of rheumatoid arthritis. Moreover, analysis of Capparis spinosa extract was performed using LC-qTOF-MS/MS.
Animals were split into six groups: negative control group, induced arthritic animals, arthritic rats receiving 7, 14 and 28 mg/kg of Capparis spinosa extract, respectively, in three groups to detect the optimum dose, and the induced group receiving a standard drug. The arthritic score was checked daily for 15 days after induction. After animals were sacrificed, their joints and muscles were subjected to microscopic and ultra-structure examinations. Ex vivo culturing of osteoclasts was performed. Cytokine levels were measured in all examined groups.
The results revealed 7 mg/kg of Capparis spinosa extract as the optimal dose, which decreased inflammation signs through controlling chondrocytes, osteoclasts, and levels of inflammatory mediators.
LC-Mass analysis revealed Capparis spinosa extract to contain a mixture of flavonol glycosides, flavan-3-ols and hydroxycinnamic acid derivatives, which may provide beneficial multifunction in regulating arthritic symptoms.
刺山柑生长于亚洲和地中海沙漠地区。刺山柑的不同部位，包括花朵，已被用于各种民间医学应用。
本研究旨在评估埃及刺山柑花朵乙醇提取物在类风湿性关节炎大鼠模型中的抗关节炎潜力。此外，使用液相色谱-四极杆飞行时间串联质谱（LC-qTOF-MS/MS）对刺山柑提取物进行分析。
将动物分为六组：阴性对照组、诱导性关节炎动物组、分别接受7、14和28mg/kg刺山柑提取物的关节炎大鼠组（共三组以检测最佳剂量）以及接受标准药物的诱导组。诱导后连续15天每天检查关节炎评分。动物处死后，对其关节和肌肉进行显微镜和超微结构检查。进行破骨细胞的体外培养。测量所有检测组中的细胞因子水平。
结果显示7mg/kg刺山柑提取物为最佳剂量，其通过控制软骨细胞、破骨细胞和炎症介质水平减轻炎症迹象。
液相色谱-质谱分析显示刺山柑提取物含有黄酮醇苷、黄烷-3-醇和羟基肉桂酸衍生物的混合物，这可能在调节关节炎症状方面提供有益的多功能作用。
