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大肠杆菌30S核糖体蛋白的三维结构。I. 蛋白质的初步分类。

Three-dimensional organization of the 30S ribosomal proteins from Escherichia coli. I. Preliminary classification of the proteins.

作者信息

Craven G R, Gupta V

出版信息

Proc Natl Acad Sci U S A. 1970 Nov;67(3):1329-36. doi: 10.1073/pnas.67.3.1329.

DOI:10.1073/pnas.67.3.1329
PMID:4922288
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC283356/
Abstract

30S ribosomal subunits from Escherichia coli were reacted with three protein reagents. After reaction, the ribosomal proteins were extracted and examined; of the 20 proteins known to compose the particle, nine did not react with any of the three reagents. We have tentatively classified these proteins as "internal," and the remaining eleven as "external." A strong correlation was found between these results and the sequence of assembly. Those proteins which enter the assembly process early are classed as internal, whereas those proteins found to participate later in the assembly sequence are classed as external.

摘要

将来自大肠杆菌的30S核糖体亚基与三种蛋白质试剂进行反应。反应后，提取并检测核糖体蛋白；在已知构成该颗粒的20种蛋白质中，有9种不与这三种试剂中的任何一种发生反应。我们暂时将这些蛋白质归类为“内部”蛋白质，其余11种归类为“外部”蛋白质。这些结果与组装顺序之间发现了很强的相关性。那些在组装过程中早期进入的蛋白质被归类为内部蛋白质，而那些在组装顺序中较晚参与的蛋白质被归类为外部蛋白质。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2245/283356/747f84e6d335/pnas00101-0251-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2245/283356/747f84e6d335/pnas00101-0251-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2245/283356/747f84e6d335/pnas00101-0251-a.jpg

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Proc Natl Acad Sci U S A. 1970 Nov;67(3):1329-36. doi: 10.1073/pnas.67.3.1329.
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本文引用的文献

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THE CHROMOSOMAL SITE SPECIFYING A RIBOSOMAL PROTEIN IN ESCHERICHIA COLI.大肠杆菌中指定核糖体蛋白的染色体位点。
Proc Natl Acad Sci U S A. 1964 Dec;52(6):1367-74. doi: 10.1073/pnas.52.6.1367.
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ABSENCE OF RIBONUCLEASE FROM THE RIBOSOMES OF PSEUDOMONAS FLUORESCENS.荧光假单胞菌核糖体中核糖核酸酶的缺失
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Reversible chemical modification of protein by 2-methoxy-5-nitrotropone. Renaturation of nitrotroponyl taka-amylase A.2-甲氧基-5-硝基环庚三烯酚酮对蛋白质的可逆化学修饰。硝基环庚三烯酚酮基高淀粉酶A的复性
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Ribosomal proteins. XXIV. Trypsin digestion as a possible probe of the conformation of Escherichia coli ribosomes.核糖体蛋白质。二十四。胰蛋白酶消化作为探测大肠杆菌核糖体构象的一种可能方法。
Mol Gen Genet. 1972;114(2):95-105. doi: 10.1007/BF00332780.
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Biochem J. 1974 Dec;143(3):607-12. doi: 10.1042/bj1430607.
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Biochemistry. 1969 Jul;8(7):2906-15. doi: 10.1021/bi00835a032.