College of Food Science and Engineering, Ocean University of China.
Department of Fishery Products, Faculty of Fisheries, Kasetsart University.
J Oleo Sci. 2022 Mar 2;71(3):401-410. doi: 10.5650/jos.ess21233. Epub 2022 Feb 11.
Liposomes are widely used as carrier system for bioactive ingredients and usually need to be stabilized by cholesterol. However, the relationship between cholesterol intake and human health has been controversial. The objective of this study was to develop novel multifunctional nanoliposomes stabilized by sea cucumber sulfated sterols via the thin-film hydration method. The liposomes obtained from this study were obviously stable for more than 27 days at 4°C. Astaxanthin was successfully encapsulated by a novel uniform liposome prepared with a mass ratio of egg yolk lecithin to sea cucumber sulfated sterols at 3:1. The mean particle size was 109.53±0.30 nm with 0.241±0.005 polydispersity index and zeta potential value of -21.13±1.01 mV. Astaxanthin-loaded liposome stabilized by sea cucumber sulfated sterols exhibited significantly higher antioxidant activities in terms of DPPH radical-scavenging activity and reducing power than the mixture of astaxanthin and blank sea cucumber sulfated sterols liposome during storage of 6 and 12 days, respectively. The in vivo digestion and absorption results showed that the bioavailability of dietary astaxanthin encapsulated in liposomes could significantly be improved. Being an efficient carrier with multifunctions, the novel liposome stabilized by sea cucumber sulfated sterols had great potential in functional food development and biomedical applications.
脂质体被广泛用作生物活性成分的载体系统，通常需要用胆固醇来稳定。然而，胆固醇摄入与人类健康之间的关系一直存在争议。本研究的目的是通过薄膜水化法开发新型多功能海参硫酸甾醇稳定的纳米脂质体。本研究获得的脂质体在 4°C 下稳定超过 27 天。通过使用蛋黄卵磷脂与海参硫酸甾醇的质量比为 3:1 制备的新型均匀脂质体成功包封虾青素。平均粒径为 109.53±0.30nm，多分散指数为 0.241±0.005，zeta 电位值为-21.13±1.01mV。与虾青素和空白海参硫酸甾醇脂质体混合物相比，在 6 和 12 天的储存期间，用海参硫酸甾醇稳定的虾青素负载脂质体在 DPPH 自由基清除活性和还原能力方面表现出显著更高的抗氧化活性。体内消化吸收结果表明，包封在脂质体中的膳食虾青素的生物利用度可以显著提高。作为一种具有多种功能的有效载体，新型海参硫酸甾醇稳定的脂质体在功能性食品开发和生物医学应用方面具有巨大的潜力。
