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芹菜素在小鼠睾丸支持细胞TM4中诱导氧化应激。

Apigenin induces oxidative stress in mouse Sertoli TM4 cells.

作者信息

Jittapalapong Sathaporn, Poompoung Thapanee, Sutjarit Samak

机构信息

Department of Veterinary Technology, Faculty of Veterinary Technology, Kasetsart University, Bangkok 10900, Thailand.

出版信息

Vet World. 2021 Dec;14(12):3132-3137. doi: 10.14202/vetworld.2021.3132-3137. Epub 2021 Dec 20.

DOI:10.14202/vetworld.2021.3132-3137
PMID:35153403
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8829396/
Abstract

BACKGROUND AND AIM

Apigenin (API) is an estrogenic compound found in many plants. Sertoli cells reside in the testis and are a key target of environmental toxicants. This study aimed to examine the cytotoxicity, especially oxidative stress of API in mouse Sertoli TM4 cells.

MATERIALS AND METHODS

Mouse Sertoli TM4 cells were treated with 50 and 100 μM API for 48 h. Cell viability, lactate dehydrogenase (LDH) activities, glutathione reductase (GR) activities, production of reactive oxygen species (ROS), and malondialdehyde (MDA) levels were evaluated using various assays.

RESULTS

Treatment with API at both 50 and 100 μM decreased viability and GR activity but increased LDH activity, ROS production, and MDA levels in mouse Sertoli TM4 cells.

CONCLUSION

Exposure to API induced oxidative stress in mouse Sertoli TM4 cells.

摘要

背景与目的

芹菜素（API）是一种存在于多种植物中的雌激素类化合物。支持细胞位于睾丸中，是环境毒物的关键作用靶点。本研究旨在检测API对小鼠支持细胞TM4细胞的细胞毒性，尤其是氧化应激。

材料与方法

用50和100μM的API处理小鼠支持细胞TM4细胞48小时。使用各种检测方法评估细胞活力、乳酸脱氢酶（LDH）活性、谷胱甘肽还原酶（GR）活性、活性氧（ROS）生成以及丙二醛（MDA）水平。

结果

50和100μM的API处理均降低了小鼠支持细胞TM4细胞的活力和GR活性，但增加了LDH活性、ROS生成及MDA水平。

结论

暴露于API可诱导小鼠支持细胞TM4细胞产生氧化应激。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e609/8829396/0b0d75a5e8ed/Vetworld-14-3132-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e609/8829396/d7c1e359e446/Vetworld-14-3132-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e609/8829396/59e02cd45836/Vetworld-14-3132-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e609/8829396/d768e2d70b86/Vetworld-14-3132-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e609/8829396/000be1209aa4/Vetworld-14-3132-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e609/8829396/0b0d75a5e8ed/Vetworld-14-3132-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e609/8829396/d7c1e359e446/Vetworld-14-3132-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e609/8829396/59e02cd45836/Vetworld-14-3132-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e609/8829396/d768e2d70b86/Vetworld-14-3132-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e609/8829396/000be1209aa4/Vetworld-14-3132-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/e609/8829396/0b0d75a5e8ed/Vetworld-14-3132-g005.jpg

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Oncol Lett. 2017 Oct;14(4):4361-4371. doi: 10.3892/ol.2017.6705. Epub 2017 Aug 2.
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Apigenin inhibits d-galactosamine/LPS-induced liver injury through upregulation of hepatic Nrf-2 and PPARγ expressions in mice.
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Apigenin-7-diglucuronide protects retinas against bright light-induced photoreceptor degeneration through the inhibition of retinal oxidative stress and inflammation.芹菜素-7-二葡萄糖醛酸苷通过抑制视网膜氧化应激和炎症反应，保护视网膜免受强光诱导的光感受器退化。
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